Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27514 | 5' | -50.4 | NC_005879.1 | + | 4617 | 0.68 | 0.786259 |
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Target: 5'- gCAGUGGUGguGgccauagCGGCUUUgAugGCCg -3' miRNA: 3'- aGUUACCACguCa------GCUGGAG-UugUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 29398 | 0.68 | 0.806738 |
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Target: 5'- aCAAcccuagGguGUCGACgUCGACACCc -3' miRNA: 3'- aGUUacca--CguCAGCUGgAGUUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 24698 | 0.68 | 0.806738 |
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Target: 5'- ----cGGaagaauagGguGUCGACgUCGACACCc -3' miRNA: 3'- aguuaCCa-------CguCAGCUGgAGUUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 33291 | 0.67 | 0.816678 |
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Target: 5'- -uGAUGG-GU-GUCGACgUCGACACa -3' miRNA: 3'- agUUACCaCGuCAGCUGgAGUUGUGg -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 7052 | 0.67 | 0.826401 |
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Target: 5'- ----aGGUGU-GUCGAUgUCGACACa -3' miRNA: 3'- aguuaCCACGuCAGCUGgAGUUGUGg -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 27480 | 0.67 | 0.835895 |
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Target: 5'- gCGAUGGUGCGGU-GGCCgauagcuuaGGCauGCCa -3' miRNA: 3'- aGUUACCACGUCAgCUGGag-------UUG--UGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 8857 | 0.66 | 0.87958 |
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Target: 5'- aCAAcuucUGGUGCGGUCaugcCCUUcACAUCa -3' miRNA: 3'- aGUU----ACCACGUCAGcu--GGAGuUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 14657 | 1 | 0.009454 |
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Target: 5'- gUCAAUGGUaCGGUCGACCUCAACACCa -3' miRNA: 3'- -AGUUACCAcGUCAGCUGGAGUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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