Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27514 | 5' | -50.4 | NC_005879.1 | + | 29319 | 0.67 | 0.816678 |
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Target: 5'- cCAGUaaaaagagGguGUCGACgUCGACACCc -3' miRNA: 3'- aGUUAcca-----CguCAGCUGgAGUUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 7052 | 0.67 | 0.826401 |
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Target: 5'- ----aGGUGU-GUCGAUgUCGACACa -3' miRNA: 3'- aguuaCCACGuCAGCUGgAGUUGUGg -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 27480 | 0.67 | 0.835895 |
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Target: 5'- gCGAUGGUGCGGU-GGCCgauagcuuaGGCauGCCa -3' miRNA: 3'- aGUUACCACGUCAgCUGGag-------UUG--UGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 15692 | 0.67 | 0.845151 |
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Target: 5'- aCAAUGGUGaauGUCGGCaaggacaAAUACCu -3' miRNA: 3'- aGUUACCACgu-CAGCUGgag----UUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 33228 | 0.66 | 0.862903 |
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Target: 5'- -----aGUGU-GUCGACgUCGACACCc -3' miRNA: 3'- aguuacCACGuCAGCUGgAGUUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 27533 | 0.66 | 0.878773 |
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Target: 5'- cCAuUGGUGCAgGUCGAgUUCAggguuuucugcguACGCUc -3' miRNA: 3'- aGUuACCACGU-CAGCUgGAGU-------------UGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 8857 | 0.66 | 0.87958 |
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Target: 5'- aCAAcuucUGGUGCGGUCaugcCCUUcACAUCa -3' miRNA: 3'- aGUU----ACCACGUCAGcu--GGAGuUGUGG- -5' |
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| 27514 | 5' | -50.4 | NC_005879.1 | + | 6978 | 0.66 | 0.895121 |
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Target: 5'- cCGuUGGUGUuaggguguGUCGACaUCGACACa -3' miRNA: 3'- aGUuACCACGu-------CAGCUGgAGUUGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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