Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27553 | 3' | -57.6 | NC_005881.1 | + | 154158 | 0.66 | 0.918461 |
Target: 5'- aCCUCCACCaCCacaaCCACCAGcagcaGCUg -3' miRNA: 3'- aGGAGGUGGaGGag--GGUGGUUag---CGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 134743 | 0.66 | 0.918461 |
Target: 5'- cUCCUCCACCaCC-CaCCACCAcUCa-- -3' miRNA: 3'- -AGGAGGUGGaGGaG-GGUGGUuAGcgg -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 173723 | 0.66 | 0.91513 |
Target: 5'- cUCCUCUuCCUCCUCUU-CCAAgauuuuaucuucugUGCCa -3' miRNA: 3'- -AGGAGGuGGAGGAGGGuGGUUa-------------GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 189796 | 0.66 | 0.912866 |
Target: 5'- ---cCCACCUCUUCUgAUCAAggUGCCa -3' miRNA: 3'- aggaGGUGGAGGAGGgUGGUUa-GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 201980 | 0.66 | 0.912866 |
Target: 5'- ---cCCACCUCUUCUgAUCAAggUGCCa -3' miRNA: 3'- aggaGGUGGAGGAGGgUGGUUa-GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 125022 | 0.66 | 0.912866 |
Target: 5'- -gUUCCACCUUCUCCUGCUGuuguAUCaCCa -3' miRNA: 3'- agGAGGUGGAGGAGGGUGGU----UAGcGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 54637 | 0.66 | 0.90705 |
Target: 5'- aCUUCUGCCcCCUCCaaugguGCCAGU-GCCa -3' miRNA: 3'- aGGAGGUGGaGGAGGg-----UGGUUAgCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 158526 | 0.66 | 0.906457 |
Target: 5'- aUCUUaCCACCUCUgacaacaCCGCCAGUggagugguugugaUGCCa -3' miRNA: 3'- -AGGA-GGUGGAGGag-----GGUGGUUA-------------GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 14756 | 0.66 | 0.894766 |
Target: 5'- uUCUUCUuCCUCCUCCgAUUccUCGUCa -3' miRNA: 3'- -AGGAGGuGGAGGAGGgUGGuuAGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 26338 | 0.67 | 0.888302 |
Target: 5'- aUUUUaacaACCUCUUCCCACCGGUCa-- -3' miRNA: 3'- aGGAGg---UGGAGGAGGGUGGUUAGcgg -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 203021 | 0.67 | 0.888302 |
Target: 5'- cUCCUCCaAUCguagUCUCUUGCCAucCGCCa -3' miRNA: 3'- -AGGAGG-UGGa---GGAGGGUGGUuaGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 188755 | 0.67 | 0.888302 |
Target: 5'- cUCCUCCaAUCguagUCUCUUGCCAucCGCCa -3' miRNA: 3'- -AGGAGG-UGGa---GGAGGGUGGUuaGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 205522 | 0.67 | 0.874748 |
Target: 5'- gUCUCCGCCUCCUuauuaucaCCCuCCucUUGCg -3' miRNA: 3'- aGGAGGUGGAGGA--------GGGuGGuuAGCGg -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 186255 | 0.67 | 0.874748 |
Target: 5'- gUCUCCGCCUCCUuauuaucaCCCuCCucUUGCg -3' miRNA: 3'- aGGAGGUGGAGGA--------GGGuGGuuAGCGg -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 206020 | 0.67 | 0.860386 |
Target: 5'- gCCuaUCUACCgCCUCCU-UCAAUUGCCa -3' miRNA: 3'- aGG--AGGUGGaGGAGGGuGGUUAGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 185756 | 0.67 | 0.860386 |
Target: 5'- gCCuaUCUACCgCCUCCU-UCAAUUGCCa -3' miRNA: 3'- aGG--AGGUGGaGGAGGGuGGUUAGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 205002 | 0.68 | 0.844483 |
Target: 5'- cCCUCC-CCacauuuuuucaaaUCUUCCCGCCuuucAUcCGCCa -3' miRNA: 3'- aGGAGGuGG-------------AGGAGGGUGGu---UA-GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 31175 | 0.68 | 0.844483 |
Target: 5'- aUUCUCCACCUggaagugagUCCCAUucuuuaaCAGUCGCCu -3' miRNA: 3'- -AGGAGGUGGAgg-------AGGGUG-------GUUAGCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 186774 | 0.68 | 0.844483 |
Target: 5'- cCCUCC-CCacauuuuuucaaaUCUUCCCGCCuuucAUcCGCCa -3' miRNA: 3'- aGGAGGuGG-------------AGGAGGGUGGu---UA-GCGG- -5' |
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27553 | 3' | -57.6 | NC_005881.1 | + | 134770 | 0.68 | 0.837423 |
Target: 5'- cUCCUCCACCcCCgccaaCCACCAcacaaGCUc -3' miRNA: 3'- -AGGAGGUGGaGGag---GGUGGUuag--CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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