Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27556 | 3' | -59.9 | NC_005882.1 | + | 12319 | 0.66 | 0.342004 |
Target: 5'- cGCGAucaGCGCGUgacggcugcCGGCGGCGCcGAUCGg -3' miRNA: 3'- -UGCU---CGCGCA---------GCCGCUGUGcCUGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 12237 | 0.68 | 0.280622 |
Target: 5'- -gGAGCGCaucaCGGCGGC-CGGugCAu -3' miRNA: 3'- ugCUCGCGca--GCCGCUGuGCCugGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 12039 | 0.67 | 0.302559 |
Target: 5'- uGCGuuGCGCGUCaGCucGGCGCGGuacGCCAGc -3' miRNA: 3'- -UGCu-CGCGCAGcCG--CUGUGCC---UGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 9546 | 0.66 | 0.366529 |
Target: 5'- cGCcAGCGCGccaaucgcagCGGCuuucaccucaagcGACGCGGACCGGu -3' miRNA: 3'- -UGcUCGCGCa---------GCCG-------------CUGUGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 3260 | 0.67 | 0.287791 |
Target: 5'- cGCGAGCGUGcgggCGGCGuCACuGuCCAGc -3' miRNA: 3'- -UGCUCGCGCa---GCCGCuGUGcCuGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 3221 | 0.69 | 0.216601 |
Target: 5'- cGCGcAGCaCGUCGGaCGACuucgcgagguACGGGCCGAu -3' miRNA: 3'- -UGC-UCGcGCAGCC-GCUG----------UGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 2024 | 1.05 | 0.000414 |
Target: 5'- uACGAGCGCGUCGGCGACACGGACCAAu -3' miRNA: 3'- -UGCUCGCGCAGCCGCUGUGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 1768 | 0.69 | 0.230727 |
Target: 5'- gACGGGauucggcguauucaGCGUgGGCG-CGCGGGCCGc -3' miRNA: 3'- -UGCUCg-------------CGCAgCCGCuGUGCCUGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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