Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27556 | 3' | -59.9 | NC_005882.1 | + | 31963 | 0.69 | 0.216601 |
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Target: 5'- gACGA--GCGUCGGCGGcCGCuGACCGAu -3' miRNA: 3'- -UGCUcgCGCAGCCGCU-GUGcCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 3221 | 0.69 | 0.216601 |
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Target: 5'- cGCGcAGCaCGUCGGaCGACuucgcgagguACGGGCCGAu -3' miRNA: 3'- -UGC-UCGcGCAGCC-GCUG----------UGCCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 21742 | 0.69 | 0.22832 |
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Target: 5'- cGCGcGCGcCGUCGccaguuCGACGCGGACCu- -3' miRNA: 3'- -UGCuCGC-GCAGCc-----GCUGUGCCUGGuu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 29736 | 0.68 | 0.246898 |
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Target: 5'- gACGAGCgGCGacacgcugCGGCuuuccGCACGGGCCGg -3' miRNA: 3'- -UGCUCG-CGCa-------GCCGc----UGUGCCUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 18284 | 0.68 | 0.266031 |
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Target: 5'- gACGAuucacauGCGCGUCGGCGGCAagaaGGcguaCAAg -3' miRNA: 3'- -UGCU-------CGCGCAGCCGCUGUg---CCug--GUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 12237 | 0.68 | 0.280622 |
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Target: 5'- -gGAGCGCaucaCGGCGGC-CGGugCAu -3' miRNA: 3'- ugCUCGCGca--GCCGCUGuGCCugGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 23236 | 0.67 | 0.287791 |
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Target: 5'- cCGGGCGCGggcCGGCGgGCAC-GAUCAAc -3' miRNA: 3'- uGCUCGCGCa--GCCGC-UGUGcCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 19806 | 0.75 | 0.08684 |
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Target: 5'- cGCGAGCGCGcCGGCGugACGacGACgGg -3' miRNA: 3'- -UGCUCGCGCaGCCGCugUGC--CUGgUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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