Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27557 | 3' | -53 | NC_005882.1 | + | 18593 | 0.66 | 0.766587 |
Target: 5'- cCUGCGCCacuuGA-UGGACG-CAgCGAaCCg -3' miRNA: 3'- -GACGCGG----CUaACCUGUaGUgGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 18968 | 0.66 | 0.766587 |
Target: 5'- -aGCGCCGAU----CGUCAUCGGccaUCCg -3' miRNA: 3'- gaCGCGGCUAaccuGUAGUGGCU---AGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 933 | 0.66 | 0.765531 |
Target: 5'- gCUG-GCgGAUcgGGGCGUCgugcaaaACCGGUCUg -3' miRNA: 3'- -GACgCGgCUAa-CCUGUAG-------UGGCUAGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 32780 | 0.66 | 0.755962 |
Target: 5'- gUGCGCagcaacgccgcuUGAUcgUGGugAUCGCgCGAUCg -3' miRNA: 3'- gACGCG------------GCUA--ACCugUAGUG-GCUAGg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 9939 | 0.66 | 0.734316 |
Target: 5'- -cGgGCCGuAUUGGcUcgCGCCGGUgCCa -3' miRNA: 3'- gaCgCGGC-UAACCuGuaGUGGCUA-GG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 14371 | 0.66 | 0.734316 |
Target: 5'- gUGCGCCGAc---ACGUCGCCcGUCa -3' miRNA: 3'- gACGCGGCUaaccUGUAGUGGcUAGg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 22373 | 0.66 | 0.731028 |
Target: 5'- uCUGCGcCCGuAUgaagcaucacuuccUGGACGucuUCGCCG-UCCu -3' miRNA: 3'- -GACGC-GGC-UA--------------ACCUGU---AGUGGCuAGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 14532 | 0.66 | 0.722216 |
Target: 5'- aCUGCGCCGAcUGGGCcaaGUUcuacagccucgcgGCCGAc-- -3' miRNA: 3'- -GACGCGGCUaACCUG---UAG-------------UGGCUagg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 23549 | 0.66 | 0.716676 |
Target: 5'- cCUGCGCCug-UGGggcaaccgcacggcgGCAUgGCCGA-CCg -3' miRNA: 3'- -GACGCGGcuaACC---------------UGUAgUGGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 28402 | 0.66 | 0.712228 |
Target: 5'- aCUGCGCCuGGUUGGcccAgGUCACUG--CCu -3' miRNA: 3'- -GACGCGG-CUAACC---UgUAGUGGCuaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 25827 | 0.67 | 0.70105 |
Target: 5'- -aGgGCCGcacGGcGCAucgucUCGCCGAUCCg -3' miRNA: 3'- gaCgCGGCuaaCC-UGU-----AGUGGCUAGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 31442 | 0.67 | 0.70105 |
Target: 5'- gCUGCGCCGGcagccGCGUCacGCCGA-CCa -3' miRNA: 3'- -GACGCGGCUaacc-UGUAG--UGGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 22696 | 0.67 | 0.699927 |
Target: 5'- aCUGCGCCGcgUcgacgucGGACAgcgacugCACCGGc-- -3' miRNA: 3'- -GACGCGGCuaA-------CCUGUa------GUGGCUagg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 11900 | 0.67 | 0.689799 |
Target: 5'- -cGaCGCCGA--GGACgAUCACCGcgCg -3' miRNA: 3'- gaC-GCGGCUaaCCUG-UAGUGGCuaGg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 21303 | 0.67 | 0.678489 |
Target: 5'- aUGCGCCGGUUGccuucggaauGGCuUgGCCGG-CCg -3' miRNA: 3'- gACGCGGCUAAC----------CUGuAgUGGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 28629 | 0.67 | 0.667131 |
Target: 5'- uUGCGCCGAUcGGACcgCAUCc---- -3' miRNA: 3'- gACGCGGCUAaCCUGuaGUGGcuagg -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 12609 | 0.67 | 0.667131 |
Target: 5'- gUGCGCCGAUcgccGCAgCGCCGG-CCg -3' miRNA: 3'- gACGCGGCUAacc-UGUaGUGGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 8238 | 0.68 | 0.644321 |
Target: 5'- -aGCGCCGGUcGGcugAUCAUCGAcaUCCc -3' miRNA: 3'- gaCGCGGCUAaCCug-UAGUGGCU--AGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 3252 | 0.68 | 0.632892 |
Target: 5'- -cGgGCCGAUcUGGAuCGUCgGCCGG-CCg -3' miRNA: 3'- gaCgCGGCUA-ACCU-GUAG-UGGCUaGG- -5' |
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27557 | 3' | -53 | NC_005882.1 | + | 20008 | 0.68 | 0.621463 |
Target: 5'- uUGCGCCGGUUauuaacGGA--UCACCGcGUUCg -3' miRNA: 3'- gACGCGGCUAA------CCUguAGUGGC-UAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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