Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27560 | 3' | -56.7 | NC_005882.1 | + | 28052 | 0.7 | 0.317859 |
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Target: 5'- -aGCUGCGCGCggCGGUgagcgcaaaaCagGCGAUGGCg -3' miRNA: 3'- cgCGACGCGUGa-GCCA----------Gg-UGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 33077 | 0.7 | 0.30224 |
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Target: 5'- aUGCUGCGCAagccCGGUCUGCGAcuGCu -3' miRNA: 3'- cGCGACGCGUga--GCCAGGUGCUacUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 2127 | 0.71 | 0.2658 |
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Target: 5'- cGCGCUGCGCcgaUUGGacaucaccgaUCCGCGcUGGCu -3' miRNA: 3'- -CGCGACGCGug-AGCC----------AGGUGCuACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 18241 | 0.71 | 0.252251 |
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Target: 5'- uCGCcGCGCAaggcCGG-CCGCGGUGGCa -3' miRNA: 3'- cGCGaCGCGUga--GCCaGGUGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 23117 | 0.72 | 0.21909 |
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Target: 5'- cGCGCcgGCGUacugcacgcagaACUCGGUCUcggucgagcuggagGCGAUGGCc -3' miRNA: 3'- -CGCGa-CGCG------------UGAGCCAGG--------------UGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 1797 | 0.73 | 0.182566 |
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Target: 5'- uCGCUGCGCAa--GGgcgaCACGGUGACg -3' miRNA: 3'- cGCGACGCGUgagCCag--GUGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 20182 | 0.74 | 0.154537 |
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Target: 5'- cGCGCUGCGCGCcCGGUCgAauCGaAUGAa -3' miRNA: 3'- -CGCGACGCGUGaGCCAGgU--GC-UACUg -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 3655 | 0.97 | 0.003178 |
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Target: 5'- cGCGCUGCGCACUCGGcUCACGAUGACc -3' miRNA: 3'- -CGCGACGCGUGAGCCaGGUGCUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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