Results 1 - 20 of 55 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27564 | 3' | -50.3 | NC_005882.1 | + | 3730 | 0.66 | 0.894813 |
Target: 5'- gGACGAAuaccuCCGGUUcaagUUCGGCCGUcucgacaaGCCg -3' miRNA: 3'- gCUGUUUc----GGCUAG----AAGUCGGCA--------UGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 14266 | 0.66 | 0.894062 |
Target: 5'- uCGACGGccgcggcGGCCG--CUUCcGCgCGUGCCu -3' miRNA: 3'- -GCUGUU-------UCGGCuaGAAGuCG-GCAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 12400 | 0.66 | 0.887165 |
Target: 5'- -aGCGuuGCCGAUCggCGccGCCGgcaGCCg -3' miRNA: 3'- gcUGUuuCGGCUAGaaGU--CGGCa--UGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 24976 | 0.66 | 0.887165 |
Target: 5'- gGAC--GGCUGaAUCUUCAGCaccaGUAgCa -3' miRNA: 3'- gCUGuuUCGGC-UAGAAGUCGg---CAUgG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 25576 | 0.66 | 0.879225 |
Target: 5'- uCGAUuguccGGCCGAgg--CGGUCGUACUg -3' miRNA: 3'- -GCUGuu---UCGGCUagaaGUCGGCAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 34293 | 0.66 | 0.879225 |
Target: 5'- gCGGucGAGCCGAUgUUCGagaaacggaucGCCGcACCa -3' miRNA: 3'- -GCUguUUCGGCUAgAAGU-----------CGGCaUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 21202 | 0.66 | 0.878415 |
Target: 5'- uCGACGAGcauGCCGAggucgagUCggacgCGGCCG-GCCa -3' miRNA: 3'- -GCUGUUU---CGGCU-------AGaa---GUCGGCaUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 13554 | 0.66 | 0.871 |
Target: 5'- aUGACGAugcGCuCGAUCUUC-GCCG-ACa -3' miRNA: 3'- -GCUGUUu--CG-GCUAGAAGuCGGCaUGg -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 16269 | 0.66 | 0.871 |
Target: 5'- cCGACGAcGUCGAUCggCAcgauguaguuGCCG-ACCu -3' miRNA: 3'- -GCUGUUuCGGCUAGaaGU----------CGGCaUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 3832 | 0.66 | 0.871 |
Target: 5'- aGcCGGAGCUGAaggUCAGCUGU-CCg -3' miRNA: 3'- gCuGUUUCGGCUagaAGUCGGCAuGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 8775 | 0.66 | 0.862497 |
Target: 5'- uGACGAAGCgaGGUCU-C-GCCGaugACCg -3' miRNA: 3'- gCUGUUUCGg-CUAGAaGuCGGCa--UGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 18357 | 0.66 | 0.854615 |
Target: 5'- -cGCAAGGCCGGUCUgaugauccugcaggaCAGCgGgcaaaugGCCg -3' miRNA: 3'- gcUGUUUCGGCUAGAa--------------GUCGgCa------UGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 13984 | 0.66 | 0.853726 |
Target: 5'- gCGGCGAAGCgGcgCUcgauacgCuGCCcGUACCg -3' miRNA: 3'- -GCUGUUUCGgCuaGAa------GuCGG-CAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 11242 | 0.67 | 0.844694 |
Target: 5'- cCGACAgcAAGCCGAauucuacggcuUCgaugagcgCAGCgCGUGCUc -3' miRNA: 3'- -GCUGU--UUCGGCU-----------AGaa------GUCG-GCAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 14083 | 0.67 | 0.844694 |
Target: 5'- cCGACGAAgacGCCGAUCca-GGCgaaCGUAUCg -3' miRNA: 3'- -GCUGUUU---CGGCUAGaagUCG---GCAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 402 | 0.67 | 0.835413 |
Target: 5'- uGGCGAGcGgCGAUCUccUCGcGCCGgACCg -3' miRNA: 3'- gCUGUUU-CgGCUAGA--AGU-CGGCaUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 11804 | 0.67 | 0.816143 |
Target: 5'- gGGCuacGAGCUGGUgUUC-GCCGcgGCCa -3' miRNA: 3'- gCUGu--UUCGGCUAgAAGuCGGCa-UGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 7338 | 0.67 | 0.816143 |
Target: 5'- uCGACAguccGAGCCGucgCUacaCAGCUcggGUACCa -3' miRNA: 3'- -GCUGU----UUCGGCua-GAa--GUCGG---CAUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 4451 | 0.67 | 0.806177 |
Target: 5'- aCGACGGGccacCCGGUCUccCAGCCGaacagcaaUACCg -3' miRNA: 3'- -GCUGUUUc---GGCUAGAa-GUCGGC--------AUGG- -5' |
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27564 | 3' | -50.3 | NC_005882.1 | + | 25724 | 0.67 | 0.806177 |
Target: 5'- cCGaACAgcGCCGAUUacgggcgCGGCCGUggcaGCCg -3' miRNA: 3'- -GC-UGUuuCGGCUAGaa-----GUCGGCA----UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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