Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27568 | 5' | -57.2 | NC_005882.1 | + | 22644 | 0.66 | 0.492165 |
Target: 5'- gAUCGGUgcggUGCCGaucaaGCCGAUcacCGCCGACu -3' miRNA: 3'- -UGGUCA----ACGGC-----CGGCUAac-GUGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 30066 | 0.66 | 0.492165 |
Target: 5'- cGCCGccuUUGCCGGCCuGGacggcgaGCGCCGAg -3' miRNA: 3'- -UGGUc--AACGGCCGG-CUaa-----CGUGGCUg -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 12677 | 0.66 | 0.492165 |
Target: 5'- gACCuucgcGUcGCCGGCaagc-GCACCGGCg -3' miRNA: 3'- -UGGu----CAaCGGCCGgcuaaCGUGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 21239 | 0.66 | 0.449853 |
Target: 5'- gACCGGUgcGCCGGCCaugaccaGGaUGaCGCCGAg -3' miRNA: 3'- -UGGUCAa-CGGCCGG-------CUaAC-GUGGCUg -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 20500 | 0.66 | 0.437851 |
Target: 5'- gACCGGaucgcUGCCGGCCGcggugaacuucucgGUcGCGCUGAg -3' miRNA: 3'- -UGGUCa----ACGGCCGGC--------------UAaCGUGGCUg -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 27148 | 0.67 | 0.430935 |
Target: 5'- gGCCuGaUGCUGGCCGGgcgcgcggUGCuCUGGCu -3' miRNA: 3'- -UGGuCaACGGCCGGCUa-------ACGuGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 2153 | 0.67 | 0.421167 |
Target: 5'- gGCCAGaucaacaCCGGCCGcgUGCucgcgcugcGCCGAUu -3' miRNA: 3'- -UGGUCaac----GGCCGGCuaACG---------UGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 31530 | 0.67 | 0.411533 |
Target: 5'- cGCUcGUcGCCGGCCaGUUGUcgAUCGACg -3' miRNA: 3'- -UGGuCAaCGGCCGGcUAACG--UGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 21623 | 0.67 | 0.392682 |
Target: 5'- aGCCAG-UGCCgGGCCaGAUUG-ACCguGACa -3' miRNA: 3'- -UGGUCaACGG-CCGG-CUAACgUGG--CUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 22390 | 0.68 | 0.38347 |
Target: 5'- uACCuGaaGCCGGCCGca-GCGCCGuCg -3' miRNA: 3'- -UGGuCaaCGGCCGGCuaaCGUGGCuG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 16287 | 0.68 | 0.38347 |
Target: 5'- aACCAGUcgGCCGGCU-----UGCCGACg -3' miRNA: 3'- -UGGUCAa-CGGCCGGcuaacGUGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 21087 | 0.68 | 0.374401 |
Target: 5'- gGCguGUgcgcaGCCGGCgCGAagGCGCuCGGCg -3' miRNA: 3'- -UGguCAa----CGGCCG-GCUaaCGUG-GCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 25893 | 0.68 | 0.374401 |
Target: 5'- uCCA--UGCCGGCagCGAccagUGUGCCGACg -3' miRNA: 3'- uGGUcaACGGCCG--GCUa---ACGUGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 12528 | 0.68 | 0.36548 |
Target: 5'- gGCCAGUgugucgcgGCggCGGCCGGc-GCugCGGCg -3' miRNA: 3'- -UGGUCAa-------CG--GCCGGCUaaCGugGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 35124 | 0.68 | 0.355837 |
Target: 5'- cCCAuucgUGCCGGCCGAUccgaUGCcuguugacgauccACUGACa -3' miRNA: 3'- uGGUca--ACGGCCGGCUA----ACG-------------UGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 31443 | 0.69 | 0.307217 |
Target: 5'- ---cGUUGCCGucgaaccuGCUGAUUGCAgCCGGCa -3' miRNA: 3'- ugguCAACGGC--------CGGCUAACGU-GGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 12444 | 0.69 | 0.307217 |
Target: 5'- gGCCGcgUGCCGGCCGGcgUUGaacCGCuCGACa -3' miRNA: 3'- -UGGUcaACGGCCGGCU--AAC---GUG-GCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 3537 | 0.7 | 0.256328 |
Target: 5'- gGCCuGggcgUGCCGGUCGucacCGCCGACg -3' miRNA: 3'- -UGGuCa---ACGGCCGGCuaacGUGGCUG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 22717 | 0.7 | 0.256328 |
Target: 5'- aGCCGGcgagcUGCgGGCCGAacUGCGCCG-Cg -3' miRNA: 3'- -UGGUCa----ACGgCCGGCUa-ACGUGGCuG- -5' |
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27568 | 5' | -57.2 | NC_005882.1 | + | 22188 | 0.7 | 0.24899 |
Target: 5'- uGCCAcagGCCGGCCGAcucgccgagaaacUUGuCGCUGACc -3' miRNA: 3'- -UGGUcaaCGGCCGGCU-------------AAC-GUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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