Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27634 | 5' | -55.3 | NC_005882.1 | + | 14873 | 0.66 | 0.610387 |
Target: 5'- aACGCGuugucGACGCcgUCgUGCGUCGAAAu -3' miRNA: 3'- -UGCGCu----CUGCGugAG-GCGCAGCUUUu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 14628 | 0.66 | 0.610387 |
Target: 5'- uCGCGuucguGAUcCGCUUCGCGUCGGGc- -3' miRNA: 3'- uGCGCu----CUGcGUGAGGCGCAGCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 19804 | 0.66 | 0.587716 |
Target: 5'- cGCGCGAG-CGCGC-CgGCGUgaCGAc-- -3' miRNA: 3'- -UGCGCUCuGCGUGaGgCGCA--GCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 7693 | 0.66 | 0.587716 |
Target: 5'- cUGuCGAGGCGCucGCgggCgGCGUCGAGGc -3' miRNA: 3'- uGC-GCUCUGCG--UGa--GgCGCAGCUUUu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 3681 | 0.66 | 0.576435 |
Target: 5'- gACGUGAucGACGCAagCgGCGUCGAc-- -3' miRNA: 3'- -UGCGCU--CUGCGUgaGgCGCAGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 3614 | 0.67 | 0.531889 |
Target: 5'- aGCGCGucGACGcCGCU-UGCGUCGAu-- -3' miRNA: 3'- -UGCGCu-CUGC-GUGAgGCGCAGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 21102 | 0.67 | 0.531889 |
Target: 5'- gGCGCGA-AgGCGCUCgGCGUugcCGAAAc -3' miRNA: 3'- -UGCGCUcUgCGUGAGgCGCA---GCUUUu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 1885 | 0.67 | 0.520938 |
Target: 5'- cCGCGAGACGC---UCGCGgccagCGAAAGu -3' miRNA: 3'- uGCGCUCUGCGugaGGCGCa----GCUUUU- -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 26631 | 0.67 | 0.520938 |
Target: 5'- cACGCGAcGGuCGCcaGCUCCGCG-CGGc-- -3' miRNA: 3'- -UGCGCU-CU-GCG--UGAGGCGCaGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 28277 | 0.67 | 0.510076 |
Target: 5'- cCGCGcAGGCGguCUCCaGCGUCa---- -3' miRNA: 3'- uGCGC-UCUGCguGAGG-CGCAGcuuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 16761 | 0.67 | 0.510076 |
Target: 5'- uGCGCG-GACGCGC---GCGUCGAc-- -3' miRNA: 3'- -UGCGCuCUGCGUGaggCGCAGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 6706 | 0.67 | 0.488646 |
Target: 5'- cACGcCGAGACGCACggCGCGcCGc--- -3' miRNA: 3'- -UGC-GCUCUGCGUGagGCGCaGCuuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 24207 | 0.68 | 0.47809 |
Target: 5'- gACGUauGGCGCACaaggCCGCGUgCGGGAAg -3' miRNA: 3'- -UGCGcuCUGCGUGa---GGCGCA-GCUUUU- -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 15951 | 0.68 | 0.467648 |
Target: 5'- aGCGCGaAGAaaucaaugcuuCGCGC-CCGCGUCGcGAu -3' miRNA: 3'- -UGCGC-UCU-----------GCGUGaGGCGCAGCuUUu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 11961 | 0.68 | 0.447124 |
Target: 5'- cCGcCGAGGCGCugGCUaCCGCGcCGAGc- -3' miRNA: 3'- uGC-GCUCUGCG--UGA-GGCGCaGCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 17203 | 0.68 | 0.441065 |
Target: 5'- gACGCG-GACGCGCUgcaggaacggcuugcCCGCG-CGAu-- -3' miRNA: 3'- -UGCGCuCUGCGUGA---------------GGCGCaGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 15760 | 0.68 | 0.437051 |
Target: 5'- uCGCGGcGGCGcCGCUCCaGCGUcCGAAu- -3' miRNA: 3'- uGCGCU-CUGC-GUGAGG-CGCA-GCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 17162 | 0.68 | 0.437051 |
Target: 5'- gGCGCGAccaGCGC-CUgCGCGUCGGc-- -3' miRNA: 3'- -UGCGCUc--UGCGuGAgGCGCAGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 20180 | 0.69 | 0.42711 |
Target: 5'- cGCGCGcuGCGCGC-CCG-GUCGAAu- -3' miRNA: 3'- -UGCGCucUGCGUGaGGCgCAGCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 34592 | 0.69 | 0.42711 |
Target: 5'- uCGCG-GAUGCGCUCaC-CGUCGGAAc -3' miRNA: 3'- uGCGCuCUGCGUGAG-GcGCAGCUUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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