Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27931 | 5' | -51.5 | NC_005886.2 | + | 1127 | 0.69 | 0.732762 |
Target: 5'- gGGCGUGAcgCGCGGgguuaaGCcUCAcGCGCGa -3' miRNA: 3'- gCUGCAUUa-GCGCC------UGuAGUuCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 3909 | 0.72 | 0.564939 |
Target: 5'- -aACGUagcGAUCGCGGuauCGUCGGGCGUu -3' miRNA: 3'- gcUGCA---UUAGCGCCu--GUAGUUCGCGc -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 5601 | 0.67 | 0.849012 |
Target: 5'- gGugGUAAUCGaCGG-UAUCAcgcugguugcgcauAGCGUGu -3' miRNA: 3'- gCugCAUUAGC-GCCuGUAGU--------------UCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 6267 | 0.77 | 0.312265 |
Target: 5'- uGGCGUAAUCGUaccggauacgucGGAUAUCAAGaCGCa -3' miRNA: 3'- gCUGCAUUAGCG------------CCUGUAGUUC-GCGc -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 7063 | 0.66 | 0.899177 |
Target: 5'- uGACGUAucCGCcGcCAcCGAGCGCGa -3' miRNA: 3'- gCUGCAUuaGCGcCuGUaGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 14927 | 0.69 | 0.743543 |
Target: 5'- -cACGUgcaacGAUCGCGaGACcuUCGAGCGCu -3' miRNA: 3'- gcUGCA-----UUAGCGC-CUGu-AGUUCGCGc -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 15137 | 0.71 | 0.598538 |
Target: 5'- uCGGCGcaccAUCGCGcGGCAUCcccGCGCGa -3' miRNA: 3'- -GCUGCau--UAGCGC-CUGUAGuu-CGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 15903 | 0.71 | 0.643709 |
Target: 5'- uCGaACGUGccGUUGCGGAacacaCGUCGgAGCGCGa -3' miRNA: 3'- -GC-UGCAU--UAGCGCCU-----GUAGU-UCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 18270 | 1.11 | 0.001781 |
Target: 5'- cCGACGUAAUCGCGGACAUCAAGCGCGg -3' miRNA: 3'- -GCUGCAUUAGCGCCUGUAGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 18862 | 0.71 | 0.643709 |
Target: 5'- gCGA-GUGuUCGCGGAaggUAAGCGCGa -3' miRNA: 3'- -GCUgCAUuAGCGCCUguaGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 19512 | 0.66 | 0.860209 |
Target: 5'- gGACGUucaacgagCGUGGuuuCAUCuuGCGCGg -3' miRNA: 3'- gCUGCAuua-----GCGCCu--GUAGuuCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 21234 | 0.67 | 0.851633 |
Target: 5'- uCGugGUGGaccgcuaCGCGcGACAacuaUCAGGUGCGu -3' miRNA: 3'- -GCugCAUUa------GCGC-CUGU----AGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 22960 | 0.66 | 0.876601 |
Target: 5'- aGACGUAGcgGCGGAUgcaAUCAAGguUGCGu -3' miRNA: 3'- gCUGCAUUagCGCCUG---UAGUUC--GCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 23631 | 0.7 | 0.676383 |
Target: 5'- uCGACGcGAUUGCguuucggGGGuCAUCGAGCGUGc -3' miRNA: 3'- -GCUGCaUUAGCG-------CCU-GUAGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 24302 | 0.66 | 0.897031 |
Target: 5'- gGGCGcggUGAuUCGUGGcgaagccgggaagaACAUCGGGCGUGg -3' miRNA: 3'- gCUGC---AUU-AGCGCC--------------UGUAGUUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 24530 | 0.67 | 0.833761 |
Target: 5'- uGACaUGGUgCGCGG-CGUCAcuggccGGCGCGc -3' miRNA: 3'- gCUGcAUUA-GCGCCuGUAGU------UCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 25346 | 0.68 | 0.764736 |
Target: 5'- gGAUGaaauuGUCGCGGAUGUCGccGCGCu -3' miRNA: 3'- gCUGCau---UAGCGCCUGUAGUu-CGCGc -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 26818 | 0.68 | 0.805303 |
Target: 5'- gGACcUGAUCGCGGcguuCGUCGAaCGCa -3' miRNA: 3'- gCUGcAUUAGCGCCu---GUAGUUcGCGc -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 29709 | 0.68 | 0.805303 |
Target: 5'- gGGCGUAccuucgccGUCGCacuucGGGCAUUcgGGGUGCGg -3' miRNA: 3'- gCUGCAU--------UAGCG-----CCUGUAG--UUCGCGC- -5' |
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27931 | 5' | -51.5 | NC_005886.2 | + | 30925 | 0.7 | 0.699828 |
Target: 5'- uCGuCGUAcGUUGUGGGCGUCAuggacguuguGGUGCGa -3' miRNA: 3'- -GCuGCAU-UAGCGCCUGUAGU----------UCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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