Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28007 | 3' | -59.8 | NC_005887.1 | + | 35404 | 0.66 | 0.426125 |
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Target: 5'- gCGCCCGcgCAcGGCCUUCGCCcacgagucgaucagGUCgaGCGa -3' miRNA: 3'- -GCGGGUa-GU-CUGGGAGCGG--------------CAGa-CGC- -5' |
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| 28007 | 3' | -59.8 | NC_005887.1 | + | 36525 | 0.66 | 0.428947 |
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Target: 5'- uCGCCCuUCGcGACCCgCGCCGaCU-CGa -3' miRNA: 3'- -GCGGGuAGU-CUGGGaGCGGCaGAcGC- -5' |
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| 28007 | 3' | -59.8 | NC_005887.1 | + | 41191 | 0.66 | 0.438434 |
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Target: 5'- cCGCCuggcugCAUCcGACCCgcgCGUCGUCgUGCc -3' miRNA: 3'- -GCGG------GUAGuCUGGGa--GCGGCAG-ACGc -5' |
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| 28007 | 3' | -59.8 | NC_005887.1 | + | 20701 | 0.66 | 0.438434 |
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Target: 5'- aCGCCCGUCAGAUUggUCGgCGUCggGUa -3' miRNA: 3'- -GCGGGUAGUCUGGg-AGCgGCAGa-CGc -5' |
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| 28007 | 3' | -59.8 | NC_005887.1 | + | 24974 | 0.66 | 0.438434 |
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Target: 5'- gCGUUCAaCAGcgaGCCCUCGCCGaacugGCGg -3' miRNA: 3'- -GCGGGUaGUC---UGGGAGCGGCaga--CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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