Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28010 | 5' | -53.3 | NC_005887.1 | + | 24226 | 0.66 | 0.743389 |
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Target: 5'- gCGCU--UCGCCGGUUg-CGUaCAGCGc -3' miRNA: 3'- aGCGAacAGCGGCUAGaaGCA-GUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 13665 | 0.66 | 0.743389 |
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Target: 5'- uUCGCgggcgUGaUCGCgGGUUgUCGUCuGGCGa -3' miRNA: 3'- -AGCGa----AC-AGCGgCUAGaAGCAG-UCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 14076 | 0.66 | 0.743389 |
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Target: 5'- gCGCUcGgcgUGCCGAUgaugcUCGUCAGUGg -3' miRNA: 3'- aGCGAaCa--GCGGCUAga---AGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 24019 | 0.66 | 0.774978 |
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Target: 5'- gUCGCaUGUCGCCGAUg--CGccccCAGUGc -3' miRNA: 3'- -AGCGaACAGCGGCUAgaaGCa---GUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 36384 | 0.66 | 0.774978 |
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Target: 5'- gUCGCUgcaUCGCCGGacg-CGcCAGCGa -3' miRNA: 3'- -AGCGAac-AGCGGCUagaaGCaGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 34383 | 1.09 | 0.00127 |
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Target: 5'- uUCGCUUGUCGCCGAUCUUCGUCAGCGc -3' miRNA: 3'- -AGCGAACAGCGGCUAGAAGCAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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