Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28010 | 5' | -53.3 | NC_005887.1 | + | 35019 | 0.74 | 0.312141 |
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Target: 5'- aCGUUUcGUCGCCaGUCgugUUGUCGGCGg -3' miRNA: 3'- aGCGAA-CAGCGGcUAGa--AGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 35055 | 0.73 | 0.353587 |
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Target: 5'- aCGCgagGUCGCUGccg-UCGUCGGCGg -3' miRNA: 3'- aGCGaa-CAGCGGCuagaAGCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 36294 | 0.66 | 0.764588 |
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Target: 5'- cUCGUccgGUUgGCCGAUCUgCG-CGGCGg -3' miRNA: 3'- -AGCGaa-CAG-CGGCUAGAaGCaGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 36384 | 0.66 | 0.774978 |
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Target: 5'- gUCGCUgcaUCGCCGGacg-CGcCAGCGa -3' miRNA: 3'- -AGCGAac-AGCGGCUagaaGCaGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 38629 | 0.69 | 0.609644 |
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Target: 5'- -aGCUUG-CGCgGAUCgcgGUCGGCGc -3' miRNA: 3'- agCGAACaGCGgCUAGaagCAGUCGC- -5' |
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| 28010 | 5' | -53.3 | NC_005887.1 | + | 39872 | 0.69 | 0.58713 |
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Target: 5'- cUCGCc-GUCGauGAUCcgCGUCAGCGc -3' miRNA: 3'- -AGCGaaCAGCggCUAGaaGCAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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