Results 1 - 20 of 65 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28014 | 5' | -52.5 | NC_005887.1 | + | 2889 | 0.66 | 0.788321 |
Target: 5'- -aCGCGCuuuUCAGGCAcgcGGCCc-UUGUc -3' miRNA: 3'- caGCGCGu--AGUCCGUu--UCGGcuAACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 31012 | 0.66 | 0.788321 |
Target: 5'- cGUgGCGCGgcgCAGGCcacuauucgcAGGCCGcggcUUGCa -3' miRNA: 3'- -CAgCGCGUa--GUCCGu---------UUCGGCu---AACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 9570 | 0.66 | 0.777919 |
Target: 5'- -gCGCGCcgAUCcuGCGcacGAGCCGGcUGCa -3' miRNA: 3'- caGCGCG--UAGucCGU---UUCGGCUaACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 23404 | 0.66 | 0.777919 |
Target: 5'- aUCGUGCGUUAuuGGCc--GCCGAcgGCc -3' miRNA: 3'- cAGCGCGUAGU--CCGuuuCGGCUaaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 26834 | 0.66 | 0.777919 |
Target: 5'- -gUGCGCGagCGGGCGcgucuGCCGGUaGCc -3' miRNA: 3'- caGCGCGUa-GUCCGUuu---CGGCUAaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 2687 | 0.66 | 0.777919 |
Target: 5'- -gUGCGCGUgaagcguaCGGGCGGcaagcGGCCGAgccGCg -3' miRNA: 3'- caGCGCGUA--------GUCCGUU-----UCGGCUaa-CG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 24343 | 0.66 | 0.771598 |
Target: 5'- -gCGCGCggcggucccauucgGUCAGGCuguuccagucggugcGGCCGAUgccgGCg -3' miRNA: 3'- caGCGCG--------------UAGUCCGuu-------------UCGGCUAa---CG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 39203 | 0.66 | 0.767351 |
Target: 5'- -gCGCgGCAUCAGGU--AGUCGAUg-- -3' miRNA: 3'- caGCG-CGUAGUCCGuuUCGGCUAacg -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 1715 | 0.66 | 0.767351 |
Target: 5'- -gCGCGCucGUCAGuGCcugucuuGCCGAUgUGCg -3' miRNA: 3'- caGCGCG--UAGUC-CGuuu----CGGCUA-ACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 29406 | 0.66 | 0.760936 |
Target: 5'- gGUCGCG-AUCAGGCucgacagcucgucGGCCGAguagucgucgGCg -3' miRNA: 3'- -CAGCGCgUAGUCCGuu-----------UCGGCUaa--------CG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 33976 | 0.66 | 0.760936 |
Target: 5'- cGUCGCGCucugcGUCAGGCuuucgcaggcuucGGCCGccagacgGCa -3' miRNA: 3'- -CAGCGCG-----UAGUCCGuu-----------UCGGCuaa----CG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 15533 | 0.66 | 0.760936 |
Target: 5'- cUCGCGCAcgcgcaggaugaaauUCagaAGGCGcucGCCGAcUGCa -3' miRNA: 3'- cAGCGCGU---------------AG---UCCGUuu-CGGCUaACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 26966 | 0.66 | 0.75663 |
Target: 5'- -gCGCGCgAUCAGcGCGgcGUCGAccUGCu -3' miRNA: 3'- caGCGCG-UAGUC-CGUuuCGGCUa-ACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 24898 | 0.66 | 0.75663 |
Target: 5'- uGUUGCGCGUCGGauucGGGCCGua-GCg -3' miRNA: 3'- -CAGCGCGUAGUCcgu-UUCGGCuaaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 17267 | 0.66 | 0.745769 |
Target: 5'- aUCGUGCcgggcgagacGUCGGGCGgcGCCGcgcGCg -3' miRNA: 3'- cAGCGCG----------UAGUCCGUuuCGGCuaaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 5506 | 0.66 | 0.745769 |
Target: 5'- cGUCGCGaucCAGcGCAAGGCUGucgaacUGCu -3' miRNA: 3'- -CAGCGCguaGUC-CGUUUCGGCua----ACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 19796 | 0.66 | 0.745769 |
Target: 5'- --aGCGUcUCGGGCAu-GUCGAUcGCg -3' miRNA: 3'- cagCGCGuAGUCCGUuuCGGCUAaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 25018 | 0.66 | 0.745769 |
Target: 5'- -gUGCGCGUCA-GCAucGCgGAagUGCa -3' miRNA: 3'- caGCGCGUAGUcCGUuuCGgCUa-ACG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 23185 | 0.67 | 0.734781 |
Target: 5'- cGUCGCGC-UC--GCGAucacGGCCGGggGCg -3' miRNA: 3'- -CAGCGCGuAGucCGUU----UCGGCUaaCG- -5' |
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28014 | 5' | -52.5 | NC_005887.1 | + | 8345 | 0.67 | 0.72368 |
Target: 5'- uUCGCGUgccGUCuGGC--GGCCGAagccUGCg -3' miRNA: 3'- cAGCGCG---UAGuCCGuuUCGGCUa---ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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