Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28014 | 5' | -52.5 | NC_005887.1 | + | 26834 | 0.66 | 0.777919 |
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Target: 5'- -gUGCGCGagCGGGCGcgucuGCCGGUaGCc -3' miRNA: 3'- caGCGCGUa-GUCCGUuu---CGGCUAaCG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 2687 | 0.66 | 0.777919 |
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Target: 5'- -gUGCGCGUgaagcguaCGGGCGGcaagcGGCCGAgccGCg -3' miRNA: 3'- caGCGCGUA--------GUCCGUU-----UCGGCUaa-CG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 9570 | 0.66 | 0.777919 |
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Target: 5'- -gCGCGCcgAUCcuGCGcacGAGCCGGcUGCa -3' miRNA: 3'- caGCGCG--UAGucCGU---UUCGGCUaACG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 2889 | 0.66 | 0.788321 |
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Target: 5'- -aCGCGCuuuUCAGGCAcgcGGCCc-UUGUc -3' miRNA: 3'- caGCGCGu--AGUCCGUu--UCGGcuAACG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 31012 | 0.66 | 0.788321 |
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Target: 5'- cGUgGCGCGgcgCAGGCcacuauucgcAGGCCGcggcUUGCa -3' miRNA: 3'- -CAgCGCGUa--GUCCGu---------UUCGGCu---AACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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