miRNA: 3'- caGCGCGUa--GuCCGU-UUCGGCUAacg -5'
miRNA: 3'- -CAGCG-----CGUAG-UCCGUUu-CGGCuaaCG- -5'
miRNA: 3'- cAGCGCGU-AGU--------CCGUUUCGGCuaaCG- -5'
miRNA: 3'- caGCGCGU------AGuCCGUUuCGGCUaaCG- -5'
miRNA: 3'- caGCGCGUa--GUCCGuUUCG-GCUaa-----CG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28014 | 5' | -52.5 | NC_005887.1 | + | 40696 | 0.67 | 0.712478 |
|
Target: 5'- -gCGCGCAcggCuGGCAgGAGCCGAUc-- -3' miRNA: 3'- caGCGCGUa--GuCCGU-UUCGGCUAacg -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 40736 | 0.69 | 0.563464 |
|
Target: 5'- gGUCGCcgcccGCGUCgAGGCGAucGCCGuugGCg -3' miRNA: 3'- -CAGCG-----CGUAG-UCCGUUu-CGGCuaaCG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 40842 | 0.68 | 0.666932 |
|
Target: 5'- uUCGUGCAgUCGgauccaccGGCGAAGCCGcc-GCg -3' miRNA: 3'- cAGCGCGU-AGU--------CCGUUUCGGCuaaCG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 40928 | 0.68 | 0.620807 |
|
Target: 5'- -gCGCGCAccgaguUC-GGCAAcGCCGAgcGCa -3' miRNA: 3'- caGCGCGU------AGuCCGUUuCGGCUaaCG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 41038 | 0.68 | 0.678404 |
|
Target: 5'- -cCGCGCGgugCAGGUcGAGCuCGAgaacaugGCg -3' miRNA: 3'- caGCGCGUa--GUCCGuUUCG-GCUaa-----CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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