miRNA: 3'- caGCGCGUAGUcCGUuuCGgCUa-ACG- -5'
miRNA: 3'- cagCGCGuAGUCCGUuuCGGCUAaCG- -5'
miRNA: 3'- cAGCGCGU-AGuCCGUuuCGGCUAa----CG- -5'
miRNA: 3'- -CAGCGCguaGUC-CGUUUCGGCua----ACG- -5'
miRNA: 3'- caGCGCGu--AGUCCGUu--UCGGcuAACG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28014 | 5' | -52.5 | NC_005887.1 | + | 25018 | 0.66 | 0.745769 |
|
Target: 5'- -gUGCGCGUCA-GCAucGCgGAagUGCa -3' miRNA: 3'- caGCGCGUAGUcCGUuuCGgCUa-ACG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 19796 | 0.66 | 0.745769 |
|
Target: 5'- --aGCGUcUCGGGCAu-GUCGAUcGCg -3' miRNA: 3'- cagCGCGuAGUCCGUuuCGGCUAaCG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 26198 | 0.67 | 0.70119 |
|
Target: 5'- cUCGCGCAcUC-GGCGc-GCCGGUgguugGCg -3' miRNA: 3'- cAGCGCGU-AGuCCGUuuCGGCUAa----CG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 5506 | 0.66 | 0.745769 |
|
Target: 5'- cGUCGCGaucCAGcGCAAGGCUGucgaacUGCu -3' miRNA: 3'- -CAGCGCguaGUC-CGUUUCGGCua----ACG- -5' |
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| 28014 | 5' | -52.5 | NC_005887.1 | + | 2889 | 0.66 | 0.788321 |
|
Target: 5'- -aCGCGCuuuUCAGGCAcgcGGCCc-UUGUc -3' miRNA: 3'- caGCGCGu--AGUCCGUu--UCGGcuAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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