Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28017 | 5' | -57.5 | NC_005887.1 | + | 36899 | 0.72 | 0.212188 |
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Target: 5'- gGUGGAUGCgGCAgGAgugcCAGCggGCGCGUg -3' miRNA: 3'- gCACCUGCG-CGUgUU----GUCGa-CGCGCA- -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 2659 | 0.72 | 0.206584 |
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Target: 5'- gGUGGGCGCGgGCGAgcacaAGCaccuggUGCGCGUg -3' miRNA: 3'- gCACCUGCGCgUGUUg----UCG------ACGCGCA- -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 14696 | 0.73 | 0.190538 |
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Target: 5'- ----cGCGCGCGCGGCAGCUcaGCGCGc -3' miRNA: 3'- gcaccUGCGCGUGUUGUCGA--CGCGCa -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 14850 | 0.74 | 0.170851 |
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Target: 5'- uCGcGGGCGUGCACAccGCAGCaacaGCGCGc -3' miRNA: 3'- -GCaCCUGCGCGUGU--UGUCGa---CGCGCa -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 41346 | 0.75 | 0.144728 |
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Target: 5'- gGUGuacagcGCGCGCACGGCGGCcggcaauuUGCGCGUg -3' miRNA: 3'- gCACc-----UGCGCGUGUUGUCG--------ACGCGCA- -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 26431 | 0.75 | 0.129393 |
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Target: 5'- gCG-GGaACGCGCGCuGCAGCcGCGCGa -3' miRNA: 3'- -GCaCC-UGCGCGUGuUGUCGaCGCGCa -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 14631 | 0.78 | 0.086827 |
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Target: 5'- --aGGGCGCGCucACGGCAGCcgGCGCGg -3' miRNA: 3'- gcaCCUGCGCG--UGUUGUCGa-CGCGCa -5' |
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| 28017 | 5' | -57.5 | NC_005887.1 | + | 31398 | 1.08 | 0.000507 |
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Target: 5'- gCGUGGACGCGCACAACAGCUGCGCGUa -3' miRNA: 3'- -GCACCUGCGCGUGUUGUCGACGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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