Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28020 | 5' | -51.2 | NC_005887.1 | + | 26150 | 0.66 | 0.893006 |
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Target: 5'- -----gCGUGUCCGgUCGC-AGCGCGg -3' miRNA: 3'- ccuaaaGCACGGGCaAGCGgUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 22772 | 0.72 | 0.529022 |
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Target: 5'- -----aCGUGCUCGUUggcucgaCGCCGGCGCGg -3' miRNA: 3'- ccuaaaGCACGGGCAA-------GCGGUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 7301 | 0.7 | 0.665661 |
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Target: 5'- uGGGUcgUCGUGUcgCCGUccgUCGCCGccgacaACACGa -3' miRNA: 3'- -CCUAa-AGCACG--GGCA---AGCGGU------UGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 35965 | 0.69 | 0.743617 |
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Target: 5'- ----cUCGUGCCC-UUCGCCc-CGCGu -3' miRNA: 3'- ccuaaAGCACGGGcAAGCGGuuGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 1594 | 0.67 | 0.805882 |
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Target: 5'- uGGGUggCG-GCCuCGaacCGCCAACGCa -3' miRNA: 3'- -CCUAaaGCaCGG-GCaa-GCGGUUGUGc -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 2172 | 0.66 | 0.86115 |
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Target: 5'- uGGAcUUCG-GCCUGa-CGCCGGcCGCGg -3' miRNA: 3'- -CCUaAAGCaCGGGCaaGCGGUU-GUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 28568 | 0.66 | 0.86115 |
|
Target: 5'- aGGcg-UCGccGCCCGcugCGCgGACGCGg -3' miRNA: 3'- -CCuaaAGCa-CGGGCaa-GCGgUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 31206 | 0.66 | 0.885452 |
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Target: 5'- aGAUUUCGggcGCCgCGUgcuucaGCaCGACGCGa -3' miRNA: 3'- cCUAAAGCa--CGG-GCAag----CG-GUUGUGC- -5' |
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| 28020 | 5' | -51.2 | NC_005887.1 | + | 34333 | 0.76 | 0.342372 |
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Target: 5'- aGGGUUUUGUG-CCGUggCGCCGGcCACGg -3' miRNA: 3'- -CCUAAAGCACgGGCAa-GCGGUU-GUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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