Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28029 | 5' | -57.4 | NC_005887.1 | + | 3471 | 0.67 | 0.438905 |
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Target: 5'- gUUCGGgccugGCGCGCUGAUgaucgagcaCGACGUCGg -3' miRNA: 3'- aAGGUCa----UGCGCGGCUG---------GCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 19103 | 0.67 | 0.448812 |
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Target: 5'- -gCCuuuUGCGCGCCGACCGcCGcCGc -3' miRNA: 3'- aaGGuc-AUGCGCGGCUGGCuGCaGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 40154 | 0.67 | 0.457832 |
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Target: 5'- -aUCGGUuuGCGCugcccgaucaccgCGGCCGGCGUCAg -3' miRNA: 3'- aaGGUCAugCGCG-------------GCUGGCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 20545 | 0.67 | 0.45884 |
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Target: 5'- -cCCGGca-GCGCCG-CgGACGUCGUg -3' miRNA: 3'- aaGGUCaugCGCGGCuGgCUGCAGUA- -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 14600 | 0.66 | 0.479248 |
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Target: 5'- -gUCGGUACGCGUaCGGCCGucggcgcCGUCGa -3' miRNA: 3'- aaGGUCAUGCGCG-GCUGGCu------GCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 14025 | 0.66 | 0.489617 |
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Target: 5'- -aCCGccgGCGCGCCG-CCGGCG-CAg -3' miRNA: 3'- aaGGUca-UGCGCGGCuGGCUGCaGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 33204 | 0.66 | 0.50009 |
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Target: 5'- gUCCAGaugaACGCGCCGcACUGGCacucgGUCGc -3' miRNA: 3'- aAGGUCa---UGCGCGGC-UGGCUG-----CAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 13192 | 0.66 | 0.50009 |
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Target: 5'- gUCgAGcagGC-CGCCGGCCGACGUg-- -3' miRNA: 3'- aAGgUCa--UGcGCGGCUGGCUGCAgua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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