Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28029 | 5' | -57.4 | NC_005887.1 | + | 14600 | 0.66 | 0.479248 |
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Target: 5'- -gUCGGUACGCGUaCGGCCGucggcgcCGUCGa -3' miRNA: 3'- aaGGUCAUGCGCG-GCUGGCu------GCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 14025 | 0.66 | 0.489617 |
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Target: 5'- -aCCGccgGCGCGCCG-CCGGCG-CAg -3' miRNA: 3'- aaGGUca-UGCGCGGCuGGCUGCaGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 13283 | 0.7 | 0.287278 |
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Target: 5'- uUUCCGGUAUGCGguugagaacgauguaCCGGCCGGCGa--- -3' miRNA: 3'- -AAGGUCAUGCGC---------------GGCUGGCUGCagua -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 13192 | 0.66 | 0.50009 |
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Target: 5'- gUCgAGcagGC-CGCCGGCCGACGUg-- -3' miRNA: 3'- aAGgUCa--UGcGCGGCUGGCUGCAgua -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 9977 | 0.72 | 0.207386 |
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Target: 5'- gUCCAcaacUACGUGUCGACgGACGUCAc -3' miRNA: 3'- aAGGUc---AUGCGCGGCUGgCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 3471 | 0.67 | 0.438905 |
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Target: 5'- gUUCGGgccugGCGCGCUGAUgaucgagcaCGACGUCGg -3' miRNA: 3'- aAGGUCa----UGCGCGGCUG---------GCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 1003 | 0.69 | 0.347246 |
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Target: 5'- -cCCGGcgGCGCGCgGAucguacucaaCCGGCGUCAc -3' miRNA: 3'- aaGGUCa-UGCGCGgCU----------GGCUGCAGUa -5' |
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| 28029 | 5' | -57.4 | NC_005887.1 | + | 740 | 0.67 | 0.429125 |
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Target: 5'- cUCaCGGgcCGCGCCGGCCGcCG-CAc -3' miRNA: 3'- aAG-GUCauGCGCGGCUGGCuGCaGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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