Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28090 | 3' | -55.4 | NC_005887.1 | + | 8706 | 0.69 | 0.454327 |
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Target: 5'- gAUCCGCCGCggCGguaAGCGCUUcgucGCGGc -3' miRNA: 3'- -UGGGUGGCGaaGC---UCGCGAAa---UGCCc -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 16240 | 0.69 | 0.454327 |
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Target: 5'- cGCCgGCCGg--CGAGCGC---ACGGGc -3' miRNA: 3'- -UGGgUGGCgaaGCUCGCGaaaUGCCC- -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 7422 | 0.69 | 0.474587 |
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Target: 5'- cACCCGCCGCcgccUCGuuuGGCGCUggcgUGCGc- -3' miRNA: 3'- -UGGGUGGCGa---AGC---UCGCGAa---AUGCcc -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 428 | 0.68 | 0.49529 |
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Target: 5'- cGCCCGCUGUUgcugCGcucgaacccucuGGCGCguguggUGCGGGc -3' miRNA: 3'- -UGGGUGGCGAa---GC------------UCGCGaa----AUGCCC- -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 40159 | 0.68 | 0.49529 |
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Target: 5'- cGCUCGCCGCgugUGAGCuGCgcgACGGc -3' miRNA: 3'- -UGGGUGGCGaa-GCUCG-CGaaaUGCCc -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 39013 | 0.67 | 0.559602 |
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Target: 5'- -gCCACCGCgcagUCGAcgGCGgCUUgcUGCGGa -3' miRNA: 3'- ugGGUGGCGa---AGCU--CGC-GAA--AUGCCc -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 17433 | 0.67 | 0.592658 |
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Target: 5'- --aCAUCGCgcucgUCGAGCGCU--ACGGc -3' miRNA: 3'- uggGUGGCGa----AGCUCGCGAaaUGCCc -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 2187 | 0.66 | 0.637164 |
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Target: 5'- cGCCgGCCGCggugaUCGGGCaGCgcaaaccgAUGGGc -3' miRNA: 3'- -UGGgUGGCGa----AGCUCG-CGaaa-----UGCCC- -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 8106 | 0.66 | 0.637164 |
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Target: 5'- aGCUCGCCGCgcucgcCGAGgcgcCGCUguuCGGGu -3' miRNA: 3'- -UGGGUGGCGaa----GCUC----GCGAaauGCCC- -5' |
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| 28090 | 3' | -55.4 | NC_005887.1 | + | 23977 | 0.82 | 0.064192 |
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Target: 5'- gGCCCACUGC-UCGAGCGCgagGCGGc -3' miRNA: 3'- -UGGGUGGCGaAGCUCGCGaaaUGCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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