Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28120 | 5' | -58.8 | NC_005887.1 | + | 17801 | 0.69 | 0.297038 |
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Target: 5'- cGCGCGGCCgugCCGAGCGUCuuGaaGAAc -3' miRNA: 3'- cCGUGCUGG---GGCUCGCAGugCgaCUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 32805 | 0.67 | 0.387039 |
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Target: 5'- cGGCGCG-CaCgCGAGCGcCGCGCUa-- -3' miRNA: 3'- -CCGUGCuG-GgGCUCGCaGUGCGAcuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 35791 | 0.67 | 0.396063 |
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Target: 5'- gGGcCACGACCgcgaCCGAGCuG-CACGCgUGGAu -3' miRNA: 3'- -CC-GUGCUGG----GGCUCG-CaGUGCG-ACUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 18210 | 0.67 | 0.405222 |
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Target: 5'- cGGCACGACgUCG-GCGgccCGCGCUu-- -3' miRNA: 3'- -CCGUGCUGgGGCuCGCa--GUGCGAcuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 11943 | 0.66 | 0.441208 |
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Target: 5'- cGGCGCugccgaacgacgaaaGACCgCCGAGCGUCGaaaacgauccgcccgUGCUGc- -3' miRNA: 3'- -CCGUG---------------CUGG-GGCUCGCAGU---------------GCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 21316 | 0.66 | 0.443152 |
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Target: 5'- cGGUGC--CCCCGAGgGUCgauGCGUUGAu -3' miRNA: 3'- -CCGUGcuGGGGCUCgCAG---UGCGACUu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 37465 | 0.66 | 0.462853 |
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Target: 5'- aGGCagucGCGACCgCGcAGCGcCA-GCUGAAg -3' miRNA: 3'- -CCG----UGCUGGgGC-UCGCaGUgCGACUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 15436 | 0.75 | 0.107168 |
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Target: 5'- cGGCGuCGACCUgcuCGGGCGUCACGUgGAAc -3' miRNA: 3'- -CCGU-GCUGGG---GCUCGCAGUGCGaCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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