Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28120 | 5' | -58.8 | NC_005887.1 | + | 42000 | 0.66 | 0.423933 |
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Target: 5'- cGGCcuGCGGCCCacguCGAGUGUCGcCGCg--- -3' miRNA: 3'- -CCG--UGCUGGG----GCUCGCAGU-GCGacuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 1000 | 0.66 | 0.423933 |
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Target: 5'- uGC-CGGCCgCCGuGCG-CGCGCUGu- -3' miRNA: 3'- cCGuGCUGG-GGCuCGCaGUGCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 5135 | 0.66 | 0.423933 |
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Target: 5'- cGGCAUGACCgCGcugcccgacgucGGCG-CGcCGCUGAu -3' miRNA: 3'- -CCGUGCUGGgGC------------UCGCaGU-GCGACUu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 26829 | 0.66 | 0.433481 |
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Target: 5'- uGCGCGugCgCGAGCGggCGCGuCUGc- -3' miRNA: 3'- cCGUGCugGgGCUCGCa-GUGC-GACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 11943 | 0.66 | 0.441208 |
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Target: 5'- cGGCGCugccgaacgacgaaaGACCgCCGAGCGUCGaaaacgauccgcccgUGCUGc- -3' miRNA: 3'- -CCGUG---------------CUGG-GGCUCGCAGU---------------GCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 21316 | 0.66 | 0.443152 |
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Target: 5'- cGGUGC--CCCCGAGgGUCgauGCGUUGAu -3' miRNA: 3'- -CCGUGcuGGGGCUCgCAG---UGCGACUu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 37465 | 0.66 | 0.462853 |
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Target: 5'- aGGCagucGCGACCgCGcAGCGcCA-GCUGAAg -3' miRNA: 3'- -CCG----UGCUGGgGC-UCGCaGUgCGACUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 33617 | 0.66 | 0.466848 |
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Target: 5'- aGCGCGGCguggucuucgaacuuCCCGuAGCG-CGCGCUGc- -3' miRNA: 3'- cCGUGCUG---------------GGGC-UCGCaGUGCGACuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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