Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28120 | 5' | -58.8 | NC_005887.1 | + | 19572 | 0.69 | 0.297038 |
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Target: 5'- aGGCACG-UCgaGcAGCGUCACGCUGu- -3' miRNA: 3'- -CCGUGCuGGggC-UCGCAGUGCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 13685 | 0.69 | 0.297038 |
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Target: 5'- aGUGCGGCCgCCGcGCGcCGCGCUGc- -3' miRNA: 3'- cCGUGCUGG-GGCuCGCaGUGCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 3444 | 0.69 | 0.273406 |
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Target: 5'- gGGCGCGACCUaccaGAGCaucgGUCuguucgggccuggcGCGCUGAu -3' miRNA: 3'- -CCGUGCUGGGg---CUCG----CAG--------------UGCGACUu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 27592 | 0.7 | 0.242383 |
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Target: 5'- aGCGCG-CCgUCGAGCGUCgugucgaagGCGCUGAu -3' miRNA: 3'- cCGUGCuGG-GGCUCGCAG---------UGCGACUu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 24063 | 0.71 | 0.212656 |
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Target: 5'- cGGCAgGAgauUCCCGAGCGccgccUCGCGCUcGAGc -3' miRNA: 3'- -CCGUgCU---GGGGCUCGC-----AGUGCGA-CUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 14204 | 0.75 | 0.110229 |
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Target: 5'- gGGCGCGGCCUCGgacagggcGGCGcCGCGCUGu- -3' miRNA: 3'- -CCGUGCUGGGGC--------UCGCaGUGCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 15436 | 0.75 | 0.107168 |
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Target: 5'- cGGCGuCGACCUgcuCGGGCGUCACGUgGAAc -3' miRNA: 3'- -CCGU-GCUGGG---GCUCGCAGUGCGaCUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 7516 | 1.08 | 0.00039 |
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Target: 5'- cGGCACGACCCCGAGCGUCACGCUGAAc -3' miRNA: 3'- -CCGUGCUGGGGCUCGCAGUGCGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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