Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28120 | 5' | -58.8 | NC_005887.1 | + | 32805 | 0.67 | 0.387039 |
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Target: 5'- cGGCGCG-CaCgCGAGCGcCGCGCUa-- -3' miRNA: 3'- -CCGUGCuG-GgGCUCGCaGUGCGAcuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 33617 | 0.66 | 0.466848 |
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Target: 5'- aGCGCGGCguggucuucgaacuuCCCGuAGCG-CGCGCUGc- -3' miRNA: 3'- cCGUGCUG---------------GGGC-UCGCaGUGCGACuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 35791 | 0.67 | 0.396063 |
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Target: 5'- gGGcCACGACCgcgaCCGAGCuG-CACGCgUGGAu -3' miRNA: 3'- -CC-GUGCUGG----GGCUCG-CaGUGCG-ACUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 36049 | 0.68 | 0.343996 |
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Target: 5'- aGGCGCaaGGCCUCGAcGCGcUCGCGCa--- -3' miRNA: 3'- -CCGUG--CUGGGGCU-CGC-AGUGCGacuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 37465 | 0.66 | 0.462853 |
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Target: 5'- aGGCagucGCGACCgCGcAGCGcCA-GCUGAAg -3' miRNA: 3'- -CCG----UGCUGGgGC-UCGCaGUgCGACUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 40362 | 0.68 | 0.360792 |
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Target: 5'- aGCGCGGCCgugCCGcuCGUCGCGCgcgagGAAa -3' miRNA: 3'- cCGUGCUGG---GGCucGCAGUGCGa----CUU- -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 41620 | 0.67 | 0.414513 |
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Target: 5'- cGGCGCGGCCCgUGAggaucugcuGCGUCuGCGCg--- -3' miRNA: 3'- -CCGUGCUGGG-GCU---------CGCAG-UGCGacuu -5' |
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| 28120 | 5' | -58.8 | NC_005887.1 | + | 42000 | 0.66 | 0.423933 |
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Target: 5'- cGGCcuGCGGCCCacguCGAGUGUCGcCGCg--- -3' miRNA: 3'- -CCG--UGCUGGG----GCUCGCAGU-GCGacuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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