Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28160 | 3' | -54.5 | NC_005887.1 | + | 31124 | 0.72 | 0.334365 |
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Target: 5'- aGCAgGGCgagCU-UGCCGGGCAGGccgUGCg -3' miRNA: 3'- -CGUgCUGaa-GGuACGGCCUGUCC---ACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 33151 | 0.66 | 0.683164 |
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Target: 5'- aGC-CGA---CCGUGCCGcGGC-GGUGCu -3' miRNA: 3'- -CGuGCUgaaGGUACGGC-CUGuCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 37413 | 0.66 | 0.705285 |
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Target: 5'- uCGCGACUgCC-UGCaCGc-CAGGUGCg -3' miRNA: 3'- cGUGCUGAaGGuACG-GCcuGUCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 37750 | 0.66 | 0.66084 |
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Target: 5'- -gGCGucGCUUgCAUGCgGGGCGGcUGCu -3' miRNA: 3'- cgUGC--UGAAgGUACGgCCUGUCcACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 38280 | 0.66 | 0.71624 |
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Target: 5'- gGCgACGACUUCgCAcGCaaCGGucaGGGUGCc -3' miRNA: 3'- -CG-UGCUGAAG-GUaCG--GCCug-UCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 40464 | 0.7 | 0.444079 |
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Target: 5'- gGCACGuCggCCGUGCCGGGCAu---- -3' miRNA: 3'- -CGUGCuGaaGGUACGGCCUGUccacg -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 41328 | 0.7 | 0.443083 |
|
Target: 5'- aGguCGACggcgCCAUugccgacGCCGaGCAGGUGCg -3' miRNA: 3'- -CguGCUGaa--GGUA-------CGGCcUGUCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 41391 | 0.68 | 0.58239 |
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Target: 5'- uGCGCGACguguucUCCGacgaUGCCGaGCAGGUc- -3' miRNA: 3'- -CGUGCUGa-----AGGU----ACGGCcUGUCCAcg -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 41649 | 0.66 | 0.705285 |
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Target: 5'- uGCGCGGCgcacgcUUCGUcuacguuggcgaGCCGGACgaAGGUaGCg -3' miRNA: 3'- -CGUGCUGa-----AGGUA------------CGGCCUG--UCCA-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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