Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28160 | 3' | -54.5 | NC_005887.1 | + | 26978 | 0.66 | 0.694256 |
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Target: 5'- -gGCGACgg-UGUGCCGGugAGGUuCa -3' miRNA: 3'- cgUGCUGaagGUACGGCCugUCCAcG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 7504 | 0.66 | 0.705285 |
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Target: 5'- uCACGACgcgcacgCCAgcccaaacgagGCgGcGGCGGGUGCg -3' miRNA: 3'- cGUGCUGaa-----GGUa----------CGgC-CUGUCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 5003 | 0.66 | 0.705285 |
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Target: 5'- uGCGCGuCUUCCAUcccgaggauCUGGAuCAGGcGCa -3' miRNA: 3'- -CGUGCuGAAGGUAc--------GGCCU-GUCCaCG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 41649 | 0.66 | 0.705285 |
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Target: 5'- uGCGCGGCgcacgcUUCGUcuacguuggcgaGCCGGACgaAGGUaGCg -3' miRNA: 3'- -CGUGCUGa-----AGGUA------------CGGCCUG--UCCA-CG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 37413 | 0.66 | 0.705285 |
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Target: 5'- uCGCGACUgCC-UGCaCGc-CAGGUGCg -3' miRNA: 3'- cGUGCUGAaGGuACG-GCcuGUCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 3946 | 0.66 | 0.71624 |
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Target: 5'- gGCAUGGCgUCgUAUGCCGGGCuGccGCc -3' miRNA: 3'- -CGUGCUGaAG-GUACGGCCUGuCcaCG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 647 | 0.66 | 0.71624 |
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Target: 5'- cGCGCGGCgagcgcacgCgCAUGaCCGccGAcCAGGUGCu -3' miRNA: 3'- -CGUGCUGaa-------G-GUAC-GGC--CU-GUCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 38280 | 0.66 | 0.71624 |
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Target: 5'- gGCgACGACUUCgCAcGCaaCGGucaGGGUGCc -3' miRNA: 3'- -CG-UGCUGAAG-GUaCG--GCCug-UCCACG- -5' |
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| 28160 | 3' | -54.5 | NC_005887.1 | + | 27398 | 0.66 | 0.71624 |
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Target: 5'- uGCGCGugGCgcagUCgAcgugGCCGGACGaGUGCg -3' miRNA: 3'- -CGUGC--UGa---AGgUa---CGGCCUGUcCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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