Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28162 | 5' | -57.3 | NC_005887.1 | + | 534 | 0.66 | 0.504059 |
Target: 5'- cGCGUCGGccGCcGcGCGCCGCGccggauaCAGCg -3' miRNA: 3'- uUGUAGCC--CGaCaCGCGGCGUa------GUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 3010 | 0.66 | 0.493482 |
Target: 5'- gAugGUCGcGGCg--GUGCCGCGgcgcuugcgCAGCu -3' miRNA: 3'- -UugUAGC-CCGacaCGCGGCGUa--------GUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 13617 | 0.66 | 0.493482 |
Target: 5'- uGGCAgcgCGGcGCgcgGCgGCCGCAcUCAGCc -3' miRNA: 3'- -UUGUa--GCC-CGacaCG-CGGCGU-AGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 4329 | 0.66 | 0.490328 |
Target: 5'- -uCAUCGGGCcgagcaguucagacUgcGUGCgGCCgaGCGUCGGCg -3' miRNA: 3'- uuGUAGCCCG--------------A--CACG-CGG--CGUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 28385 | 0.66 | 0.481965 |
Target: 5'- cGGCuUCGcGGCUGUcgaucacGCGCaCGUcgCAGCc -3' miRNA: 3'- -UUGuAGC-CCGACA-------CGCG-GCGuaGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 13502 | 0.66 | 0.472641 |
Target: 5'- gAACGUgcUGaGCgGUGCGCCGCucgucgCAGCa -3' miRNA: 3'- -UUGUA--GCcCGaCACGCGGCGua----GUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 14105 | 0.66 | 0.462388 |
Target: 5'- cGGCAUCGucucaGGCUGcgccgGCGgCGCGcCGGCg -3' miRNA: 3'- -UUGUAGC-----CCGACa----CGCgGCGUaGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 37374 | 0.67 | 0.456292 |
Target: 5'- cAGCGUCGGcuuccuucuccuucaGCUG-GCGCUGCG-CGGUc -3' miRNA: 3'- -UUGUAGCC---------------CGACaCGCGGCGUaGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 35475 | 0.67 | 0.452253 |
Target: 5'- cGACucgUGGGCgaaggccGUGCGCgggCGCAUCgAGCa -3' miRNA: 3'- -UUGua-GCCCGa------CACGCG---GCGUAG-UCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 40514 | 0.67 | 0.452253 |
Target: 5'- cGGCAuUCGGGaucaUGUcgaGCGCCGauuugAUCAGCu -3' miRNA: 3'- -UUGU-AGCCCg---ACA---CGCGGCg----UAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 481 | 0.67 | 0.442241 |
Target: 5'- cAACAgCGGGCgGcGCGgCGCGUCuucGCg -3' miRNA: 3'- -UUGUaGCCCGaCaCGCgGCGUAGu--CG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 34917 | 0.67 | 0.441246 |
Target: 5'- gAACGUCGcGCUGUGCggaacuuGCCGuCGcCGGCu -3' miRNA: 3'- -UUGUAGCcCGACACG-------CGGC-GUaGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 17966 | 0.67 | 0.432355 |
Target: 5'- uGCG-CGGGCU---CGCCGgCAUCGGCc -3' miRNA: 3'- uUGUaGCCCGAcacGCGGC-GUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 17790 | 0.67 | 0.422599 |
Target: 5'- --gGUCGuGGCUGU-CGUCGCAggGGCu -3' miRNA: 3'- uugUAGC-CCGACAcGCGGCGUagUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 1232 | 0.67 | 0.412977 |
Target: 5'- ----gCGGGCgcUGCGCgGCGcUCAGCa -3' miRNA: 3'- uuguaGCCCGacACGCGgCGU-AGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 316 | 0.68 | 0.394144 |
Target: 5'- cGGCAUCaGGUcgagGCGCCGCAggucaaaCAGCa -3' miRNA: 3'- -UUGUAGcCCGaca-CGCGGCGUa------GUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 38645 | 0.68 | 0.384939 |
Target: 5'- cAACAUCGGGCcGUcguucuCGUCG-AUCAGCa -3' miRNA: 3'- -UUGUAGCCCGaCAc-----GCGGCgUAGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 28264 | 0.68 | 0.36696 |
Target: 5'- aGACGcgCGGcGCUGaUGCGgCGCA-CAGCc -3' miRNA: 3'- -UUGUa-GCC-CGAC-ACGCgGCGUaGUCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 18482 | 0.68 | 0.36696 |
Target: 5'- cAGCGUCGuGCUGacgacgGCGCCGCGgcguaGGCc -3' miRNA: 3'- -UUGUAGCcCGACa-----CGCGGCGUag---UCG- -5' |
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28162 | 5' | -57.3 | NC_005887.1 | + | 32299 | 0.69 | 0.324595 |
Target: 5'- -uCGUCGGGCcagccgGcGCGCCGCAgu-GCg -3' miRNA: 3'- uuGUAGCCCGa-----CaCGCGGCGUaguCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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