Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28165 | 5' | -50.5 | NC_005887.1 | + | 13105 | 0.68 | 0.737136 |
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Target: 5'- -aACACCUAACACGaacggagaacGACGUGCAu--- -3' miRNA: 3'- uaUGUGGAUUGUGC----------CUGCAUGUcguu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 7839 | 0.68 | 0.748384 |
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Target: 5'- cUGCgGCCUG--AUGGGCGUGCAGUAc -3' miRNA: 3'- uAUG-UGGAUugUGCCUGCAUGUCGUu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 31266 | 0.67 | 0.770457 |
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Target: 5'- gAUGCGCC--ACGCGGAauCGUuugaGCAGCGg -3' miRNA: 3'- -UAUGUGGauUGUGCCU--GCA----UGUCGUu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 21390 | 0.67 | 0.812509 |
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Target: 5'- -aGCGCC-AGCGCGGGa--ACAGCGAg -3' miRNA: 3'- uaUGUGGaUUGUGCCUgcaUGUCGUU- -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 24225 | 0.66 | 0.841794 |
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Target: 5'- -cGCGCUUcGC-CGGuuGCGUACAGCGc -3' miRNA: 3'- uaUGUGGAuUGuGCC--UGCAUGUCGUu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 25343 | 0.66 | 0.841794 |
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Target: 5'- -aGCACCUugcucuGCGCGuGGCGgauCGGCAc -3' miRNA: 3'- uaUGUGGAu-----UGUGC-CUGCau-GUCGUu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 25017 | 0.66 | 0.85106 |
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Target: 5'- uGUGCGCgUcagcauCGCGGAaGUGCAGCAc -3' miRNA: 3'- -UAUGUGgAuu----GUGCCUgCAUGUCGUu -5' |
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| 28165 | 5' | -50.5 | NC_005887.1 | + | 21328 | 0.74 | 0.388768 |
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Target: 5'- --uCGCCUGAC-CGGACGUugAGCc- -3' miRNA: 3'- uauGUGGAUUGuGCCUGCAugUCGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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