Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28174 | 5' | -56.9 | NC_005887.1 | + | 33975 | 0.66 | 0.524039 |
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Target: 5'- --cGUCCGUCAGCaccggCGCCGUaUCa -3' miRNA: 3'- gaaCGGGCGGUCGguaa-GCGGCA-AGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 34099 | 0.66 | 0.534754 |
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Target: 5'- --aGCCUGCauauCGGUCAggaugUUGCCGUUCu -3' miRNA: 3'- gaaCGGGCG----GUCGGUa----AGCGGCAAGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 34616 | 1.08 | 0.000577 |
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Target: 5'- cCUUGCCCGCCAGCCAUUCGCCGUUCGc -3' miRNA: 3'- -GAACGGGCGGUCGGUAAGCGGCAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 35405 | 0.67 | 0.492415 |
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Target: 5'- --cGCCCGCgcaCGGCC-UUCGCCcacgagUCGa -3' miRNA: 3'- gaaCGGGCG---GUCGGuAAGCGGca----AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 36954 | 0.68 | 0.410043 |
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Target: 5'- --cGCCCGCCGGUuCAccggucgcgcuggcUUCGCC-UUCGa -3' miRNA: 3'- gaaCGGGCGGUCG-GU--------------AAGCGGcAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 37756 | 0.69 | 0.385147 |
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Target: 5'- --aGCCCGCCcaacuuGCCGU-CGUCGUUgCGc -3' miRNA: 3'- gaaCGGGCGGu-----CGGUAaGCGGCAA-GC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 39035 | 0.66 | 0.571713 |
|
Target: 5'- gUUGCCCGUcuuCAGCCGaugccagagcugcgUCGCCGgcgUGa -3' miRNA: 3'- gAACGGGCG---GUCGGUa-------------AGCGGCaa-GC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 39715 | 0.71 | 0.272856 |
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Target: 5'- -gUGCCCGCC-GCCAccucauccUCGCgCGUUCu -3' miRNA: 3'- gaACGGGCGGuCGGUa-------AGCG-GCAAGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 40401 | 0.68 | 0.403493 |
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Target: 5'- -aUGCCCgGCaCGGCCGacgUGCCGgUCGa -3' miRNA: 3'- gaACGGG-CG-GUCGGUaa-GCGGCaAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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