Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28174 | 5' | -56.9 | NC_005887.1 | + | 14094 | 0.69 | 0.376183 |
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Target: 5'- -aUGCUCGUCAGUgGUgaagCGCCGcUCGc -3' miRNA: 3'- gaACGGGCGGUCGgUAa---GCGGCaAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 14014 | 0.69 | 0.394251 |
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Target: 5'- -aUGCCgCGCCGGCgCAgaCGCCGa--- -3' miRNA: 3'- gaACGG-GCGGUCG-GUaaGCGGCaagc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 36954 | 0.68 | 0.410043 |
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Target: 5'- --cGCCCGCCGGUuCAccggucgcgcuggcUUCGCC-UUCGa -3' miRNA: 3'- gaaCGGGCGGUCG-GU--------------AAGCGGcAAGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 25480 | 0.67 | 0.4617 |
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Target: 5'- gUUGCCUGUCgAGCCGUccgCGCCG--CGg -3' miRNA: 3'- gAACGGGCGG-UCGGUAa--GCGGCaaGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 28967 | 0.66 | 0.510234 |
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Target: 5'- --cGCCgCGCCGGCCuUcagcgcgagcgccaUCGCCGggCu -3' miRNA: 3'- gaaCGG-GCGGUCGGuA--------------AGCGGCaaGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 9927 | 0.66 | 0.534754 |
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Target: 5'- --cG-CCGCCGGCCAcaUCGCCGccggccacgUCGa -3' miRNA: 3'- gaaCgGGCGGUCGGUa-AGCGGCa--------AGC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 34099 | 0.66 | 0.534754 |
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Target: 5'- --aGCCUGCauauCGGUCAggaugUUGCCGUUCu -3' miRNA: 3'- gaaCGGGCG----GUCGGUa----AGCGGCAAGc -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 39035 | 0.66 | 0.571713 |
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Target: 5'- gUUGCCCGUcuuCAGCCGaugccagagcugcgUCGCCGgcgUGa -3' miRNA: 3'- gAACGGGCG---GUCGGUa-------------AGCGGCaa-GC- -5' |
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| 28174 | 5' | -56.9 | NC_005887.1 | + | 20195 | 0.72 | 0.233413 |
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Target: 5'- uUUGCgCGUCAGCCcUUCGCCGa--- -3' miRNA: 3'- gAACGgGCGGUCGGuAAGCGGCaagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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