Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28207 | 3' | -52.9 | NC_005891.1 | + | 29279 | 0.98 | 0.008007 |
Target: 5'- aGGGUGUCGACGUCGACACCCUAg--- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUgaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 10513 | 0.66 | 0.775524 |
Target: 5'- cGGUGUCGGCGUCuccuuuGgAgCCUGCUc- -3' miRNA: 3'- cCCACAGCUGCAGc-----UgUgGGAUGAau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 29606 | 0.66 | 0.775524 |
Target: 5'- uGGGUGUUGAgGcguuUCGACACUUUgauuugcgACUUAg -3' miRNA: 3'- -CCCACAGCUgC----AGCUGUGGGA--------UGAAU- -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 20205 | 0.66 | 0.743762 |
Target: 5'- cGGGUGaCGACGgucUGcCACCUUACa-- -3' miRNA: 3'- -CCCACaGCUGCa--GCuGUGGGAUGaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 14587 | 0.69 | 0.597876 |
Target: 5'- uGGUGUUGAgGUCGACcgcaccauugacGCCCUAg--- -3' miRNA: 3'- cCCACAGCUgCAGCUG------------UGGGAUgaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 1092 | 0.73 | 0.378699 |
Target: 5'- -uGUGUCGACGUCGACACUauucccgaUGCa-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGGg-------AUGaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 37918 | 0.74 | 0.318407 |
Target: 5'- gGGGUGuUCGAcCGUCGACACCaUugUUc -3' miRNA: 3'- -CCCAC-AGCU-GCAGCUGUGGgAugAAu -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 36170 | 0.76 | 0.232418 |
Target: 5'- -uGUGUCGACGUCGACACCgccuUUGCg-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGG----GAUGaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 33179 | 0.82 | 0.105328 |
Target: 5'- -uGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGGGaugaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 16833 | 0.85 | 0.059981 |
Target: 5'- aGGGUGUCGACGUCGACACaCCcgaacaggucgUGCUa- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUG-GG-----------AUGAau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 34707 | 0.93 | 0.017262 |
Target: 5'- aGGGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 21747 | 0.93 | 0.017262 |
Target: 5'- aGGGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 24578 | 1 | 0.005532 |
Target: 5'- uGGGUGUCGACGUCGACACCCUAUUc- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 6989 | 0.76 | 0.232418 |
Target: 5'- gGGuGUGUCGACaUCGACACaCCUAUUUu -3' miRNA: 3'- -CC-CACAGCUGcAGCUGUG-GGAUGAAu -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 34767 | 1.09 | 0.001219 |
Target: 5'- uGGGUGUCGACGUCGACACCCUACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAAU- -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 21807 | 1.06 | 0.002261 |
Target: 5'- uGGGUGUCGACGUCGACACCCUACUg- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 29339 | 0.97 | 0.00889 |
Target: 5'- aGGGUGUCGACGUCGACACCCUcuuuuuacuggagagACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGA---------------UGAAU- -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 24638 | 0.94 | 0.014808 |
Target: 5'- aGGGUGUCGACGUCGACACCC-ACa-- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaUGaau -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 16893 | 0.88 | 0.040539 |
Target: 5'- gGGuGUGUCGACGUCGACACCCUcccccucagGCUUc -3' miRNA: 3'- -CC-CACAGCUGCAGCUGUGGGA---------UGAAu -5' |
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28207 | 3' | -52.9 | NC_005891.1 | + | 33237 | 0.84 | 0.069651 |
Target: 5'- uGGGUGUCGACGUCGACACaCUGgUa- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGgGAUgAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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