Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28207 | 3' | -52.9 | NC_005891.1 | + | 7051 | 0.78 | 0.186615 |
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Target: 5'- aGGuGUGUCGAUGUCGACacACCCUAa--- -3' miRNA: 3'- -CC-CACAGCUGCAGCUG--UGGGAUgaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 37979 | 0.72 | 0.426216 |
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Target: 5'- uGGUGUCGACgGUCGACACaCCc----- -3' miRNA: 3'- cCCACAGCUG-CAGCUGUG-GGaugaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 1148 | 0.69 | 0.5753 |
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Target: 5'- -aGUGUCGACGUCGACACa------- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGggaugaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 22549 | 0.68 | 0.620577 |
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Target: 5'- gGGGUuccCGACGUgGACGCCUU-CUUGg -3' miRNA: 3'- -CCCAca-GCUGCAgCUGUGGGAuGAAU- -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 34163 | 0.68 | 0.643321 |
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Target: 5'- uGGGUGUUGuAUGUCGAaaCACCCcaaggGCa-- -3' miRNA: 3'- -CCCACAGC-UGCAGCU--GUGGGa----UGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 9012 | 0.68 | 0.677325 |
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Target: 5'- -uGUGUCGAUGUCGACAUaCUuccGCUUu -3' miRNA: 3'- ccCACAGCUGCAGCUGUGgGA---UGAAu -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 34767 | 1.09 | 0.001219 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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