Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28207 | 3' | -52.9 | NC_005891.1 | + | 36170 | 0.76 | 0.232418 |
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Target: 5'- -uGUGUCGACGUCGACACCgccuUUGCg-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGG----GAUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 6989 | 0.76 | 0.232418 |
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Target: 5'- gGGuGUGUCGACaUCGACACaCCUAUUUu -3' miRNA: 3'- -CC-CACAGCUGcAGCUGUG-GGAUGAAu -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 37918 | 0.74 | 0.318407 |
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Target: 5'- gGGGUGuUCGAcCGUCGACACCaUugUUc -3' miRNA: 3'- -CCCAC-AGCU-GCAGCUGUGGgAugAAu -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 1092 | 0.73 | 0.378699 |
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Target: 5'- -uGUGUCGACGUCGACACUauucccgaUGCa-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGGg-------AUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 14587 | 0.69 | 0.597876 |
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Target: 5'- uGGUGUUGAgGUCGACcgcaccauugacGCCCUAg--- -3' miRNA: 3'- cCCACAGCUgCAGCUG------------UGGGAUgaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 20205 | 0.66 | 0.743762 |
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Target: 5'- cGGGUGaCGACGgucUGcCACCUUACa-- -3' miRNA: 3'- -CCCACaGCUGCa--GCuGUGGGAUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 24578 | 1 | 0.005532 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUAUUc- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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