Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28207 | 3' | -52.9 | NC_005891.1 | + | 34163 | 0.68 | 0.643321 |
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Target: 5'- uGGGUGUUGuAUGUCGAaaCACCCcaaggGCa-- -3' miRNA: 3'- -CCCACAGC-UGCAGCU--GUGGGa----UGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 34707 | 0.93 | 0.017262 |
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Target: 5'- aGGGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 34767 | 1.09 | 0.001219 |
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Target: 5'- uGGGUGUCGACGUCGACACCCUACUUAa -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGAUGAAU- -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 36170 | 0.76 | 0.232418 |
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Target: 5'- -uGUGUCGACGUCGACACCgccuUUGCg-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGG----GAUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 36227 | 0.84 | 0.071758 |
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Target: 5'- cGGUGUCGACGUCGACACaCUGCc-- -3' miRNA: 3'- cCCACAGCUGCAGCUGUGgGAUGaau -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 37918 | 0.74 | 0.318407 |
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Target: 5'- gGGGUGuUCGAcCGUCGACACCaUugUUc -3' miRNA: 3'- -CCCAC-AGCU-GCAGCUGUGGgAugAAu -5' |
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| 28207 | 3' | -52.9 | NC_005891.1 | + | 37979 | 0.72 | 0.426216 |
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Target: 5'- uGGUGUCGACgGUCGACACaCCc----- -3' miRNA: 3'- cCCACAGCUG-CAGCUGUG-GGaugaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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