Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28253 | 5' | -48.9 | NC_005902.1 | + | 52303 | 0.67 | 0.998584 |
Target: 5'- aCAAUgcuuAGCAUCaUUAaugaGGAGUUAACCCUu -3' miRNA: 3'- aGUUG----UCGUGG-AGU----UCUCAAUUGGGA- -5' |
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28253 | 5' | -48.9 | NC_005902.1 | + | 172702 | 0.66 | 0.999044 |
Target: 5'- gUCAAUgaUGCCUUAGGGGUUAACUUUa -3' miRNA: 3'- -AGUUGucGUGGAGUUCUCAAUUGGGA- -5' |
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28253 | 5' | -48.9 | NC_005902.1 | + | 26474 | 0.66 | 0.999222 |
Target: 5'- aCAACAGCAU----GGAGUUAAUCUUa -3' miRNA: 3'- aGUUGUCGUGgaguUCUCAAUUGGGA- -5' |
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28253 | 5' | -48.9 | NC_005902.1 | + | 1595 | 0.66 | 0.999222 |
Target: 5'- aUUAACAaCACCUUAAGAGUUAAa--- -3' miRNA: 3'- -AGUUGUcGUGGAGUUCUCAAUUggga -5' |
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28253 | 5' | -48.9 | NC_005902.1 | + | 24091 | 0.66 | 0.99937 |
Target: 5'- aUUAACGaUGCCUUAGGAGUUAAaguguuuacCCCUa -3' miRNA: 3'- -AGUUGUcGUGGAGUUCUCAAUU---------GGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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