Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28268 | 5' | -41.7 | NC_005902.1 | + | 158173 | 0.75 | 0.998801 |
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Target: 5'- -aACAaGUAUaAGCCUUAAGAUUUAAa -3' miRNA: 3'- agUGUaCAUAcUCGGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 43812 | 0.74 | 0.999379 |
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Target: 5'- aUACGUGUuugcuaugcucGUGcaAGCCUUAGGAUUUAAa -3' miRNA: 3'- aGUGUACA-----------UAC--UCGGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 12699 | 0.73 | 0.999821 |
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Target: 5'- -gACA-GUAUaAGCCUUAAGAUUUAAc -3' miRNA: 3'- agUGUaCAUAcUCGGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 163485 | 0.72 | 0.999923 |
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Target: 5'- -aACAaGUAU-AGCCUUAGGAUUUAAa -3' miRNA: 3'- agUGUaCAUAcUCGGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 100313 | 0.72 | 0.99997 |
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Target: 5'- uUCACucuUGUAU-AGaCCUUAAGAUUUAAa -3' miRNA: 3'- -AGUGu--ACAUAcUC-GGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 91209 | 0.71 | 0.999978 |
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Target: 5'- cUCACuUGUAgaAGCCUUAgaGGAUUUAAa -3' miRNA: 3'- -AGUGuACAUacUCGGAAU--UCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 10342 | 0.71 | 0.999992 |
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Target: 5'- uUUACucGUAUaAGCCUUAGGAUUUAAa -3' miRNA: 3'- -AGUGuaCAUAcUCGGAAUUCUAAAUU- -5' |
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| 28268 | 5' | -41.7 | NC_005902.1 | + | 149721 | 0.83 | 0.909833 |
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Target: 5'- cUCACuUGUAUaAGCCUUAGGAUUUAAa -3' miRNA: 3'- -AGUGuACAUAcUCGGAAUUCUAAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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