Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28305 | 3' | -53.1 | NC_005905.1 | + | 8914 | 0.82 | 0.16696 |
|
Target: 5'- uUGGGCaGAGUUAGCUUCGACAAAUUUu -3' miRNA: 3'- -ACCCGgUUCGAUCGGAGCUGUUUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8849 | 1.03 | 0.006397 |
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Target: 5'- uUGGGCCAAGCUAGCUUCGACAGAUCUc -3' miRNA: 3'- -ACCCGGUUCGAUCGGAGCUGUUUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8804 | 0.98 | 0.014284 |
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Target: 5'- uUGGGCCAAGCUAGCUUCGACAAAUUUu -3' miRNA: 3'- -ACCCGGUUCGAUCGGAGCUGUUUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8788 | 0.68 | 0.811632 |
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Target: 5'- uUGGcccaaGCCGGGCUGGaCUCGAUAAAcCUa -3' miRNA: 3'- -ACC-----CGGUUCGAUCgGAGCUGUUUaGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8740 | 0.94 | 0.026596 |
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Target: 5'- uUGGGCCAAGCUAGCUUCGACA-AUCUc -3' miRNA: 3'- -ACCCGGUUCGAUCGGAGCUGUuUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8723 | 0.66 | 0.901671 |
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Target: 5'- uUGGcCCAAGCcGGCCUgGACAc---- -3' miRNA: 3'- -ACCcGGUUCGaUCGGAgCUGUuuaga -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8695 | 0.98 | 0.014284 |
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Target: 5'- uUGGGCCAAGCUAGCUUCGACAAAUUUu -3' miRNA: 3'- -ACCCGGUUCGAUCGGAGCUGUUUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8630 | 1.03 | 0.006591 |
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Target: 5'- uUGGGCCAAGCUAGCUUCGACAAAUCUc -3' miRNA: 3'- -ACCCGGUUCGAUCGGAGCUGUUUAGA- -5' |
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| 28305 | 3' | -53.1 | NC_005905.1 | + | 8585 | 0.68 | 0.811632 |
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Target: 5'- uUGGaCCGAuuUAGCUUCGACAAAUUUu -3' miRNA: 3'- -ACCcGGUUcgAUCGGAGCUGUUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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