Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28485 | 5' | -65.9 | NC_005946.1 | + | 86233 | 0.66 | 0.399956 |
Target: 5'- aGGCCGAGa--GGGCCUCgggUCAGuuGGGCa -3' miRNA: 3'- -CCGGCUCccgCCCGGGG---AGUCc-UCCG- -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 103584 | 0.66 | 0.403223 |
Target: 5'- cGGCCGGGGaGCucgugagcaGGGUgUCCUCGgacgcggaccccgccGGGGGCu -3' miRNA: 3'- -CCGGCUCC-CG---------CCCG-GGGAGU---------------CCUCCG- -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 38199 | 0.66 | 0.41646 |
Target: 5'- gGGCUGAGGGacguCGGGcCCCCUCucGAc-- -3' miRNA: 3'- -CCGGCUCCC----GCCC-GGGGAGucCUccg -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 93097 | 0.66 | 0.41646 |
Target: 5'- gGGCCGAGGuGaC-GGCUgg-CAGGAGGUa -3' miRNA: 3'- -CCGGCUCC-C-GcCCGGggaGUCCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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