Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28485 | 5' | -65.9 | NC_005946.1 | + | 32647 | 0.71 | 0.200236 |
Target: 5'- uGGCCaacaaGAGGGUggacgccaccGGGCCCCUCAucgccuuccuccuGGAcgGGCu -3' miRNA: 3'- -CCGG-----CUCCCG----------CCCGGGGAGU-------------CCU--CCG- -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 49841 | 0.71 | 0.191386 |
Target: 5'- aGGgUGAGGGU-GGCCCUgucuacguUCAGGAGGg -3' miRNA: 3'- -CCgGCUCCCGcCCGGGG--------AGUCCUCCg -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 103440 | 0.73 | 0.132879 |
Target: 5'- gGGaCuCGAGGG-GGGCCCCUUAcGuGAGGUg -3' miRNA: 3'- -CC-G-GCUCCCgCCCGGGGAGU-C-CUCCG- -5' |
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28485 | 5' | -65.9 | NC_005946.1 | + | 33057 | 0.74 | 0.116478 |
Target: 5'- uGGCCGAggGGGCGGGgUUCUCccuggagacacccaGGGAGGUu -3' miRNA: 3'- -CCGGCU--CCCGCCCgGGGAG--------------UCCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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