Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28724 | 5' | -47.8 | NC_006146.1 | + | 79075 | 0.67 | 0.999366 |
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Target: 5'- --gGACGCAuGCGAUgacggcugcCAaCUUGCGCUg -3' miRNA: 3'- uugCUGCGUuUGUUA---------GUaGAACGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 54874 | 0.66 | 0.99948 |
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Target: 5'- aAugGGCuGCGGGCGuagcgggGUCcgCUcGCGCCg -3' miRNA: 3'- -UugCUG-CGUUUGU-------UAGuaGAaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 685 | 0.66 | 0.999492 |
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Target: 5'- aGAUGGCGCaAGAUGAUUGUUU-GCGUCg -3' miRNA: 3'- -UUGCUGCG-UUUGUUAGUAGAaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 156844 | 0.66 | 0.999595 |
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Target: 5'- gGAgGAcCGCAGcCAGcCGgcgCUUGCGCCu -3' miRNA: 3'- -UUgCU-GCGUUuGUUaGUa--GAACGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 116505 | 0.66 | 0.999672 |
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Target: 5'- gAGCGGCGCGAGgAggcggcaccacccAUCGUCUgGC-CCa -3' miRNA: 3'- -UUGCUGCGUUUgU-------------UAGUAGAaCGcGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 95321 | 0.66 | 0.999679 |
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Target: 5'- --aGGCGCGGAgGGUCugggagagAUUUUGCGCg -3' miRNA: 3'- uugCUGCGUUUgUUAG--------UAGAACGCGg -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 153348 | 0.66 | 0.999798 |
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Target: 5'- uGGCGGCGCAGGgAGUCAuaggcaaauuggaUCUggaugGUgGCCg -3' miRNA: 3'- -UUGCUGCGUUUgUUAGU-------------AGAa----CG-CGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 84188 | 0.66 | 0.999803 |
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Target: 5'- uAGCGAUGCuuGCuc-CAUCgcGUGCCc -3' miRNA: 3'- -UUGCUGCGuuUGuuaGUAGaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 111093 | 0.66 | 0.999803 |
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Target: 5'- aGACGACGCccuCAAUCAgCUggaGCucuGCCg -3' miRNA: 3'- -UUGCUGCGuuuGUUAGUaGAa--CG---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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