Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28724 | 5' | -47.8 | NC_006146.1 | + | 84188 | 0.66 | 0.999803 |
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Target: 5'- uAGCGAUGCuuGCuc-CAUCgcGUGCCc -3' miRNA: 3'- -UUGCUGCGuuUGuuaGUAGaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 79075 | 0.67 | 0.999366 |
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Target: 5'- --gGACGCAuGCGAUgacggcugcCAaCUUGCGCUg -3' miRNA: 3'- uugCUGCGUuUGUUA---------GUaGAACGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 68070 | 0.69 | 0.99431 |
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Target: 5'- gAGCGGCGCGAGCAAgcgCAgagCccccGCGCg -3' miRNA: 3'- -UUGCUGCGUUUGUUa--GUa--Gaa--CGCGg -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 54874 | 0.66 | 0.99948 |
|
Target: 5'- aAugGGCuGCGGGCGuagcgggGUCcgCUcGCGCCg -3' miRNA: 3'- -UugCUG-CGUUUGU-------UAGuaGAaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 54837 | 0.67 | 0.999215 |
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Target: 5'- gGACGuCGCc-ACAuUUGUCaUGCGCCa -3' miRNA: 3'- -UUGCuGCGuuUGUuAGUAGaACGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 50287 | 0.69 | 0.995125 |
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Target: 5'- aAGgGGCGCAGGaGAUCuGUCagGCGCCg -3' miRNA: 3'- -UUgCUGCGUUUgUUAG-UAGaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 32061 | 0.67 | 0.999199 |
|
Target: 5'- gGGCGugGCGAGCAucgGUCAgCUgaccaaaUGUGCa -3' miRNA: 3'- -UUGCugCGUUUGU---UAGUaGA-------ACGCGg -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 22462 | 0.7 | 0.989885 |
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Target: 5'- gGACGAUGCcuGCAccagAUCAUacaGCGCCu -3' miRNA: 3'- -UUGCUGCGuuUGU----UAGUAgaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 685 | 0.66 | 0.999492 |
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Target: 5'- aGAUGGCGCaAGAUGAUUGUUU-GCGUCg -3' miRNA: 3'- -UUGCUGCG-UUUGUUAGUAGAaCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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