Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28724 | 5' | -47.8 | NC_006146.1 | + | 54874 | 0.66 | 0.99948 |
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Target: 5'- aAugGGCuGCGGGCGuagcgggGUCcgCUcGCGCCg -3' miRNA: 3'- -UugCUG-CGUUUGU-------UAGuaGAaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 160458 | 0.67 | 0.999199 |
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Target: 5'- aGGCGGCGCucuCGugGUCAUCcagguagUUGCGCa -3' miRNA: 3'- -UUGCUGCGuuuGU--UAGUAG-------AACGCGg -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 155428 | 0.67 | 0.998818 |
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Target: 5'- cACG-CGCAGGCAcagcgucucgGUCAgggucucgcUCUgcUGCGCCa -3' miRNA: 3'- uUGCuGCGUUUGU----------UAGU---------AGA--ACGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 125310 | 0.68 | 0.997908 |
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Target: 5'- gGGCGugGUAAAUu-UCAUCUgGCauGCCa -3' miRNA: 3'- -UUGCugCGUUUGuuAGUAGAaCG--CGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 104169 | 0.69 | 0.996473 |
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Target: 5'- gAGCGACuGCAGAucCAGUCGcagggccgUCU-GCGCCc -3' miRNA: 3'- -UUGCUG-CGUUU--GUUAGU--------AGAaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 147852 | 0.69 | 0.995125 |
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Target: 5'- aGGCGACGUugcaGAUCAg---GCGCCa -3' miRNA: 3'- -UUGCUGCGuuugUUAGUagaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 50287 | 0.69 | 0.995125 |
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Target: 5'- aAGgGGCGCAGGaGAUCuGUCagGCGCCg -3' miRNA: 3'- -UUgCUGCGUUUgUUAG-UAGaaCGCGG- -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 68070 | 0.69 | 0.99431 |
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Target: 5'- gAGCGGCGCGAGCAAgcgCAgagCccccGCGCg -3' miRNA: 3'- -UUGCUGCGUUUGUUa--GUa--Gaa--CGCGg -5' |
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| 28724 | 5' | -47.8 | NC_006146.1 | + | 111093 | 0.66 | 0.999803 |
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Target: 5'- aGACGACGCccuCAAUCAgCUggaGCucuGCCg -3' miRNA: 3'- -UUGCUGCGuuuGUUAGUaGAa--CG---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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