Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28731 | 3' | -57.1 | NC_006146.1 | + | 152858 | 0.67 | 0.858466 |
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Target: 5'- -gGCGGaGCCGGCGaUGA-CGGuGGAGg -3' miRNA: 3'- gaCGUCaUGGCCGC-ACUaGUCcCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 149780 | 0.67 | 0.858466 |
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Target: 5'- -gGCGGaGCCGGCGaUGA-CGGuGGAGg -3' miRNA: 3'- gaCGUCaUGGCCGC-ACUaGUCcCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 143625 | 0.67 | 0.858466 |
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Target: 5'- -gGCGGaGCCGGCGaUGA-CGGuGGAGg -3' miRNA: 3'- gaCGUCaUGGCCGC-ACUaGUCcCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 50710 | 0.67 | 0.850775 |
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Target: 5'- -gGCAGgccugaaaGCCGGCGuUGAgCGGGGGcGa -3' miRNA: 3'- gaCGUCa-------UGGCCGC-ACUaGUCCCCuC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 14619 | 0.67 | 0.850775 |
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Target: 5'- gUGCccUGCUGGCGcUGcgUGGGGGAGc -3' miRNA: 3'- gACGucAUGGCCGC-ACuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 106645 | 0.68 | 0.809563 |
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Target: 5'- cCUGCuGcgGCCGGC-UGGUCAGGGc-- -3' miRNA: 3'- -GACGuCa-UGGCCGcACUAGUCCCcuc -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 37653 | 0.69 | 0.745543 |
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Target: 5'- uUGguGUAUUGGUGuUGAgggUAGGGGGGc -3' miRNA: 3'- gACguCAUGGCCGC-ACUa--GUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 43599 | 0.7 | 0.66676 |
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Target: 5'- -gGCGGgagGCCGGguCG-GGUCGGGGGAa -3' miRNA: 3'- gaCGUCa--UGGCC--GCaCUAGUCCCCUc -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 44779 | 0.66 | 0.900253 |
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Target: 5'- gUGCcGUGCCGG-Gc--UCAGGGGGGc -3' miRNA: 3'- gACGuCAUGGCCgCacuAGUCCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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