Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28731 | 3' | -57.1 | NC_006146.1 | + | 149780 | 0.67 | 0.858466 |
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Target: 5'- -gGCGGaGCCGGCGaUGA-CGGuGGAGg -3' miRNA: 3'- gaCGUCaUGGCCGC-ACUaGUCcCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 166665 | 0.66 | 0.865959 |
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Target: 5'- -aGCGGgaagggggAgCGGUGUGGccuggCAGGGGGGa -3' miRNA: 3'- gaCGUCa-------UgGCCGCACUa----GUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 170661 | 0.66 | 0.865959 |
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Target: 5'- -cGCAG-AUCGGCG----CGGGGGAGg -3' miRNA: 3'- gaCGUCaUGGCCGCacuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 88256 | 0.66 | 0.887187 |
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Target: 5'- uUGCAGgcaaaauCCGGCGUGugcuggGUCGGGccucuGGGGa -3' miRNA: 3'- gACGUCau-----GGCCGCAC------UAGUCC-----CCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 71484 | 0.66 | 0.887187 |
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Target: 5'- cCUGCcGUuugGCCGGUgGUGccUGGGGGAGg -3' miRNA: 3'- -GACGuCA---UGGCCG-CACuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 52990 | 0.66 | 0.887187 |
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Target: 5'- -gGCGGaccUGCCGGcCGUGGccgCGGGGGu- -3' miRNA: 3'- gaCGUC---AUGGCC-GCACUa--GUCCCCuc -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 44002 | 0.66 | 0.893831 |
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Target: 5'- -aGUAG-GCCGGagGUgGAUguGGGGAGg -3' miRNA: 3'- gaCGUCaUGGCCg-CA-CUAguCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 128868 | 0.66 | 0.893831 |
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Target: 5'- -aGCuc-ACCGGCGUGAgcccgggccCAGaGGGAGu -3' miRNA: 3'- gaCGucaUGGCCGCACUa--------GUC-CCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 44779 | 0.66 | 0.900253 |
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Target: 5'- gUGCcGUGCCGG-Gc--UCAGGGGGGc -3' miRNA: 3'- gACGuCAUGGCCgCacuAGUCCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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