Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28731 | 3' | -57.1 | NC_006146.1 | + | 34029 | 0.67 | 0.858466 |
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Target: 5'- -gGCAG-GCgGGCGUGGUCccGGGGc -3' miRNA: 3'- gaCGUCaUGgCCGCACUAGucCCCUc -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 102572 | 0.7 | 0.68679 |
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Target: 5'- -aGCAGguccgaGCCGaGCGUGcagGUCGGGGGGu -3' miRNA: 3'- gaCGUCa-----UGGC-CGCAC---UAGUCCCCUc -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 66507 | 0.71 | 0.640567 |
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Target: 5'- aUGCAccgGCCGGCGUGAUaCacgccgcugucaaacAGGGGGGc -3' miRNA: 3'- gACGUca-UGGCCGCACUA-G---------------UCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 117565 | 0.71 | 0.60627 |
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Target: 5'- gCUGUucagGGUauggGCCGGCGUGuggaGUCGGGGGuGa -3' miRNA: 3'- -GACG----UCA----UGGCCGCAC----UAGUCCCCuC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 170093 | 0.73 | 0.526872 |
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Target: 5'- -gGCGGgagggGCCGGCGccg-CAGGGGGGg -3' miRNA: 3'- gaCGUCa----UGGCCGCacuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 169161 | 0.73 | 0.507582 |
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Target: 5'- -gGCGGgagggGCCGGCGccugCAGGGGGGg -3' miRNA: 3'- gaCGUCa----UGGCCGCacuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 168229 | 0.73 | 0.507582 |
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Target: 5'- -gGCGGgagggGCCGGCGccugCAGGGGGGg -3' miRNA: 3'- gaCGUCa----UGGCCGCacuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 167297 | 0.73 | 0.507582 |
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Target: 5'- -gGCGGgagggGCCGGCGccugCAGGGGGGg -3' miRNA: 3'- gaCGUCa----UGGCCGCacuaGUCCCCUC- -5' |
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| 28731 | 3' | -57.1 | NC_006146.1 | + | 71484 | 0.66 | 0.887187 |
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Target: 5'- cCUGCcGUuugGCCGGUgGUGccUGGGGGAGg -3' miRNA: 3'- -GACGuCA---UGGCCG-CACuaGUCCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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