Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2874 | 3' | -55.3 | NC_001493.1 | + | 102300 | 0.66 | 0.939984 |
Target: 5'- gCUCCccCUCGGuGCUCAGGGcCGccuguGCCCg -3' miRNA: 3'- gGAGGc-GAGUC-UGAGUCUCuGC-----UGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 102123 | 0.66 | 0.939984 |
Target: 5'- --aUCGgUCGGGCgggCAcAGGCGGCCCu -3' miRNA: 3'- ggaGGCgAGUCUGa--GUcUCUGCUGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 97097 | 0.66 | 0.935182 |
Target: 5'- cCCggCCGUUaucgaaaaaaCAGAauugCGGGGACGACUCg -3' miRNA: 3'- -GGa-GGCGA----------GUCUga--GUCUCUGCUGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 52909 | 0.66 | 0.935182 |
Target: 5'- cCCUCgGagUCAGuCUCGaGGACGaACCCc -3' miRNA: 3'- -GGAGgCg-AGUCuGAGUcUCUGC-UGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 130987 | 0.66 | 0.930143 |
Target: 5'- gUUCCGgaCGGGgaguucugcUUCGGGGACGGCgCCu -3' miRNA: 3'- gGAGGCgaGUCU---------GAGUCUCUGCUG-GG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 54554 | 0.66 | 0.930143 |
Target: 5'- aUCUgUGCUUGuGGCUguGAGGCGAUCg -3' miRNA: 3'- -GGAgGCGAGU-CUGAguCUCUGCUGGg -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 15433 | 0.66 | 0.930143 |
Target: 5'- gUUCCGgaCGGGgaguucugcUUCGGGGACGGCgCCu -3' miRNA: 3'- gGAGGCgaGUCU---------GAGUCUCUGCUG-GG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 28243 | 0.66 | 0.927006 |
Target: 5'- -gUCUGCUCGcggaguacgagagacGACUgGGuAGACGGCCg -3' miRNA: 3'- ggAGGCGAGU---------------CUGAgUC-UCUGCUGGg -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 21401 | 0.66 | 0.924866 |
Target: 5'- uUCUCCcgucaucaCUCGGuCUCcGAGGCGuCCCg -3' miRNA: 3'- -GGAGGc-------GAGUCuGAGuCUCUGCuGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 64400 | 0.66 | 0.924326 |
Target: 5'- aCCga-GCUCAucacgacaacgguGGCUCGGAuGAgGACCCc -3' miRNA: 3'- -GGaggCGAGU-------------CUGAGUCU-CUgCUGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 132494 | 0.66 | 0.919352 |
Target: 5'- --gCC-CUCGGGgUCAcaucGAGAUGGCCCa -3' miRNA: 3'- ggaGGcGAGUCUgAGU----CUCUGCUGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 16940 | 0.66 | 0.919352 |
Target: 5'- --gCC-CUCGGGgUCAcaucGAGAUGGCCCa -3' miRNA: 3'- ggaGGcGAGUCUgAGU----CUCUGCUGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 124885 | 0.66 | 0.913601 |
Target: 5'- uCCUCCGCggaGGACgUGGAGcgcguggacGCGuCCCg -3' miRNA: 3'- -GGAGGCGag-UCUGaGUCUC---------UGCuGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 90824 | 0.66 | 0.913601 |
Target: 5'- -gUCUGCUCGaacggccucucGACUCGGAGccgGCGGgCCu -3' miRNA: 3'- ggAGGCGAGU-----------CUGAGUCUC---UGCUgGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 9331 | 0.66 | 0.913601 |
Target: 5'- uCCUCCGCggaGGACgUGGAGcgcguggacGCGuCCCg -3' miRNA: 3'- -GGAGGCGag-UCUGaGUCUC---------UGCuGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 10005 | 0.66 | 0.913013 |
Target: 5'- gCCUCCGCguagcucgguggcUgGGACgauauggugagCAGGGAUGcGCCCg -3' miRNA: 3'- -GGAGGCG-------------AgUCUGa----------GUCUCUGC-UGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 125559 | 0.66 | 0.913013 |
Target: 5'- gCCUCCGCguagcucgguggcUgGGACgauauggugagCAGGGAUGcGCCCg -3' miRNA: 3'- -GGAGGCG-------------AgUCUGa----------GUCUCUGC-UGGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 53863 | 0.67 | 0.907613 |
Target: 5'- gCUuuGCUCGGuuucuaGCUCGGgagcccGGGCGAgCCa -3' miRNA: 3'- gGAggCGAGUC------UGAGUC------UCUGCUgGG- -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 20556 | 0.67 | 0.888258 |
Target: 5'- aCCUggcaGCUCGGGCUCAucaugcucGAGACGAUa- -3' miRNA: 3'- -GGAgg--CGAGUCUGAGU--------CUCUGCUGgg -5' |
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2874 | 3' | -55.3 | NC_001493.1 | + | 33434 | 0.67 | 0.888258 |
Target: 5'- gCUCuCGUUCGGug-CGGGGACGGUCCg -3' miRNA: 3'- gGAG-GCGAGUCugaGUCUCUGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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