Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28763 | 3' | -52 | NC_006146.1 | + | 156309 | 0.66 | 0.976901 |
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Target: 5'- aGAGGUGAUGuagaggcgcggguccGGGAuGGGGCUcACg-- -3' miRNA: 3'- gUUCUACUAC---------------CCCU-CCCCGAuUGaaa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 134596 | 0.66 | 0.978377 |
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Target: 5'- --uGAUuGUGGGGGGaGGGCUcuCUUa -3' miRNA: 3'- guuCUAcUACCCCUC-CCCGAuuGAAa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 42553 | 0.66 | 0.980679 |
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Target: 5'- -cGGggGGUGGGGGGGcGCUGAa--- -3' miRNA: 3'- guUCuaCUACCCCUCCcCGAUUgaaa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 51465 | 0.66 | 0.982794 |
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Target: 5'- aGAGGUguggaGAUGGGcGGGGGCUGcgGCg-- -3' miRNA: 3'- gUUCUA-----CUACCCcUCCCCGAU--UGaaa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 48225 | 0.66 | 0.982794 |
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Target: 5'- --cGAUGAgaaGGAGGGGCUgcaGACUa- -3' miRNA: 3'- guuCUACUaccCCUCCCCGA---UUGAaa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 41303 | 0.66 | 0.984729 |
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Target: 5'- --cGGUGA-GGGGcAGGGGCUuuuucagagggAGCUg- -3' miRNA: 3'- guuCUACUaCCCC-UCCCCGA-----------UUGAaa -5' |
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| 28763 | 3' | -52 | NC_006146.1 | + | 164062 | 0.66 | 0.984729 |
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Target: 5'- gGGGGUGAa-GGGAGGGGCa------ -3' miRNA: 3'- gUUCUACUacCCCUCCCCGauugaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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